We narrowed to 2,435 results for: LRE

1. pR6Kg-Alpha2

   Plasmid

   #165863

   Purpose

   Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS697

   Plasmid

   #168778

   Purpose

   Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Type

   Empty backbone

   Use

   Tags

   Cmyc and HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Eomes gRNA#1

   Plasmid

   #170834

   Purpose

   Cas9-mediated knockout of Eomes in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Eomes (Eomes Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Eomes gRNA#2

   Plasmid

   #170835

   Purpose

   Cas9-mediated knockout of Eomes in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Eomes (Eomes Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Eomes gRNA#3

   Plasmid

   #170836

   Purpose

   Cas9-mediated knockout of Eomes in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Eomes (Eomes Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcry:mCherry,-600unc:cct3GFP

   Plasmid

   #172528

   Purpose

   alphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.

   Depositor

   Joachim Berger

   Article

   Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

   Insert

   cct3-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPuroR-GMRWhite

   Plasmid

   #165884

   Purpose

   Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Transgenesis Ready Selection Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-mSNX27-H112A RNAi-resistant

   Plasmid

   #163621

   Purpose

   mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H112A and RNAi resistance

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Donor-PIGAshort

   Plasmid

   #153547

   Purpose

   Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 603-bp fragment from intron 5 and exon 6)
   

   Use

   Donor plasmid

   Tags

   N/A

   Expression

   Mutation

   No mutation

   Promoter

   No promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Donor-PIGAmut

    Plasmid

    #153548

    Purpose

    Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
    

    Use

    Donor plasmid

    Tags

    N/A

    Expression

    Mutation

    a 3-bp mutation in exon 6

    Promoter

    No promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pISH-OMACS

    Plasmid

    #136765

    Purpose

    Template to synthesize riboprobes for in situ hybridization (ISH) for Acsm4

    Depositor

    Peter Mombaerts

    Article

    Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

    Insert

    Acsm4 (Acsm4 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    ISH Probe insert, A102T- please see depositor com…

    Promoter

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC_h7SK M15 (UUA)

    Plasmid

    #154785

    Purpose

    plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    UUA anticodon and G10A, G14U, G16U, C20U, C52A

    Promoter

    h7SK

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. miR-223-RFP pCDNA

    Plasmid

    #97142

    Purpose

    expression of miR-223 and TagRFP

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    miR-223
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSI-check2-TAB2-3'UTR

    Plasmid

    #97157

    Purpose

    For dual luciferase report assay

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    TAB2 3'UTR (TAB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2-cryR;snx5CE1-basEgfp

    Plasmid

    #90138

    Purpose

    contains snx5 enhancer sequence and a basal promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    snx5E1basEgfp (snx5 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2-cryR;yrkCE1-P1Egfp

    Plasmid

    #90156

    Purpose

    contains yrk enhancer sequence, and yrk specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    yrkE1P1Egfp (yrk Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2-cryR;snx5CE1-P1Egfp

    Plasmid

    #90152

    Purpose

    contains snx5 enhancer sequence, and snx5 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    snx5E1P1Egfp (snx5 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2-cryR;yrkCE1-basEgfp

    Plasmid

    #90142

    Purpose

    contains yrk enhancer sequence and a basal promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    yrkE1basEgfp (yrk Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. FgH1tUTG_muBim_Ex3

    Plasmid

    #85533

    Purpose

    Inducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu Bim
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Lyn-FRB-DMVF-181-N

    Plasmid

    #87609

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Lyn-FRB-DMVF181-N
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3-LifeAct-FRB-DMVF-181-N

    Plasmid

    #87610

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-DMVF181-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eGFP-KIF5B

    Plasmid

    #172203

    Purpose

    Expresses KIF5B tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJRH051

    Plasmid

    #171625

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCMV-Parkin

    Plasmid

    #213547

    Purpose

    Transient mammalian expresssion of untagged Parkin

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPBbsr-JNK KTR-mCherry

    Plasmid

    #115493

    Purpose

    Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

    Insert

    JNK KTR-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry G229L, mCherry Y298 deleted

    Promoter

    CAG

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. keratin 8 (krt8)-H2B-RFP

    Plasmid

    #105955

    Purpose

    transgenesis

    Depositor

    Caren Norden

    Article

    Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

    Insert

    krt8 (KRT8 Human)
    

    Use

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMK292 (mAID-mCherry2-NeoR)

    Plasmid

    #72830

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Neo
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. -5.5kb-UCP1-GFP

    Plasmid

    #104585

    Purpose

    Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

    Depositor

    Irfan Lodhi

    Article

    Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

    Insert

    UCP1 promoter (Ucp1 Mouse)
    

    Use

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UCP1

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-TORCAR

    Plasmid

    #64927

    Purpose

    FRET-based biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR (EIF4EBP1 Synthetic, Human)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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