We narrowed to 32,486 results for: Eng;

1. CoV2-Spike-D614G-D1118H

   Plasmid

   #177974

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, D1118H)

   Depositor

   Melanie Ott

   Article

   Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

   Insert

   SARS-CoV-2 Spike protein (D614G, D1118H) (S )
   

   Expression

   Mammalian

   Mutation

   D614G, D1118H

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pANY8

   Plasmid

   #164900

   Purpose

   Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Tags

   His-ELP-Intein-tag

   Expression

   Bacterial

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pANY4-pL18-ccdB

   Plasmid

   #164898

   Purpose

   Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15b-12x

   Plasmid

   #61699

   Purpose

   Expresses PTDH mutant Opt12x

   Depositor

   Huimin Zhao

   Article

   Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

   Insert

   Opt12x
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pANY4-pR18/pL18-ccdB

   Plasmid

   #164899

   Purpose

   Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJKW 0633

   Plasmid

   #74122

   Purpose

   Photinus pyralis sulfotransferase ST2 - pHis8-4

   Depositor

   Jing-Ke Weng

   Article

   Fallon et al Biochemistry. 2016 May 26.

   Insert

   Photinus pyralis sulfotransferase 2
   

   Tags

   8x-His, TEV protease site (ENLYFQ)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CoV2-Spike-D614G-Alpha

   Plasmid

   #177961

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)

   Depositor

   Melanie Ott

   Article

   Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

   Insert

   SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
   

   Expression

   Mammalian

   Mutation

   del69, 70, del144, D614G, N501Y, A570D, P681H, T7…

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAKTaq

   Plasmid

   #25712

   Purpose

   Bacterial expression of Taq polymerase

   Depositor

   David Engelke

   Article

   Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

   Insert

   Taq polymerase
   

   Expression

   Bacterial

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BioBrick pET-24a(+)

   Plasmid

   #117802

   Purpose

   BioBrick pET vector for protein overexpression in E. coli

   Depositor

   Anthony Forster

   Article

   Du et al Biotechnol Bioeng. 2009 Dec 15;104(6):11

   Type

   Empty backbone

   Use

   Escherichia coli

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJKW 0690

    Plasmid

    #74123

    Purpose

    Photinus pyralis sulfotransferase ST3 - pHis8-4

    Depositor

    Jing-Ke Weng

    Article

    Fallon et al Biochemistry. 2016 May 26.

    Insert

    Photinus pyralis sulfotransferase 3
    

    Tags

    8x-His,TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVP226

    Plasmid

    #222056

    Purpose

    GreenGate AE bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pVP227

    Plasmid

    #222057

    Purpose

    GreenGate AF bridge module containing filller sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pVP228

    Plasmid

    #222058

    Purpose

    GreenGate BD bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pVP229

    Plasmid

    #222059

    Purpose

    GreenGate BE bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pVP230

    Plasmid

    #222060

    Purpose

    GreenGate CD bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pVP231

    Plasmid

    #222061

    Purpose

    GreenGate CE bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pVP295

    Plasmid

    #222064

    Purpose

    GreenGate AB base entry vector with an Esp3I domesticated backbone.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    Domesticated of Esp3I sites

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pVP296

    Plasmid

    #222065

    Purpose

    GreenGate BC base entry vector with an Esp3I domesticated backbone.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    Domesticated of Esp3I sites

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pVP297

    Plasmid

    #222066

    Purpose

    GreenGate CD base entry vector with an Esp3I domesticated backbone.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    Domesticated of Esp3I sites

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pVP298

    Plasmid

    #222067

    Purpose

    GreenGate DE base entry vector with an Esp3I domesticated backbone.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    Domesticated of Esp3I sites

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pVP299

    Plasmid

    #222068

    Purpose

    GreenGate EF base entry vector with an Esp3I domesticated backbone.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    Domesticated of Esp3I sites

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pVP300

    Plasmid

    #222069

    Purpose

    GreenGate FG base entry vector with an Esp3I domesticated backbone.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    Domesticated of Esp3I sites

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pVP333

    Plasmid

    #222044

    Purpose

    GreenGate AB bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pVP334

    Plasmid

    #222045

    Purpose

    GreenGate FG bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pVP120

    Plasmid

    #222046

    Purpose

    GreenGate BG bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pVP121

    Plasmid

    #222047

    Purpose

    GreenGate CG bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pVP122

    Plasmid

    #222048

    Purpose

    GreenGate DG bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pVP123

    Plasmid

    #222049

    Purpose

    GreenGate EG bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pVP183

    Plasmid

    #222050

    Purpose

    GreenGate DF bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pVP184

    Plasmid

    #222051

    Purpose

    GreenGate EF bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pVP185

    Plasmid

    #222052

    Purpose

    GreenGate BF bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pVP186

    Plasmid

    #222053

    Purpose

    GreenGate CF bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pVP224

    Plasmid

    #222054

    Purpose

    GreenGate AC bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pVP225

    Plasmid

    #222055

    Purpose

    GreenGate AD bridge module containing filler sequence.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Insert

    Filler
    

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pVP089

    Plasmid

    #222040

    Purpose

    MultiGreen 1.0 base HG entry vector.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    H and G overhangs for GreenGate assembly

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pVP091

    Plasmid

    #222042

    Purpose

    MultiGreen 1.0 base FH entry vector.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    F and H overhangs for GreenGate assembly

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pGGPK-AG2

    Plasmid

    #222027

    Purpose

    Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pRSETA_ionRD

    Plasmid

    #172924

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSCBE3

    Plasmid

    #133966

    Purpose

    Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    BE3
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJH110

    Plasmid

    #68376

    Purpose

    Clone cassette to replace RFP for integration into C31 landing pad strain

    Depositor

    Trevor Charles

    Article

    Heil et al J Vis Exp. 2012 Mar 16;(61). pii: 3698.

    Type

    Empty backbone

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSCBE2

    Plasmid

    #133965

    Purpose

    Expresses BE2 CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    BE2
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRSETA_ionRE

    Plasmid

    #172926

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBdxs

    Plasmid

    #17821

    Depositor

    Jay Keasling

    Article

    Kim et al Biotechnol Bioeng. 2001 Feb 20. 72(4):40

    Insert

    dxs
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

44. pJKW 0643

    Plasmid

    #74121

    Purpose

    Photinus pyralis luciferin sulfotransferase LST - pHis8-4

    Depositor

    Jing-Ke Weng

    Article

    Fallon et al Biochemistry. 2016 May 26.

    Insert

    Photinus pyralis firefly luciferin sulfotransferase
    

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRSETA_ionKE

    Plasmid

    #172925

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorKE
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. BBa_J72214-BBa_J72090

    Plasmid

    #40782

    Depositor

    Christopher Anderson

    Article

    Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

    Insert

    VioABCDE
    

    Use

    Synthetic Biology; Phagemid

    Expression

    Bacterial

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3.1_ionRD

    Plasmid

    #172931

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Hook1-EGFP

    Plasmid

    #171941

    Purpose

    Expresses Hook1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Hook1
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3.1_ionRE

    Plasmid

    #172932

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. BBa_J72113-BBa_J72152

    Plasmid

    #40784

    Depositor

    Christopher Anderson

    Article

    Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

    Insert

    Phagemid + GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge