We narrowed to 23,628 results for: CRISPR

1. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

   Plasmid

   #192676

   Purpose

   Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

   Plasmid

   #114726

   Purpose

   dCas9-VP160 expression in mammalian cells

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   dCas9-VP160
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIV489

   Plasmid

   #177697

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJZC523

   Plasmid

   #62320

   Purpose

   sgRNA for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6_(GLuc)_TOP1

   Plasmid

   #68423

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sg-tdTomato90/816

   Plasmid

   #179918

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPPC017.109

   Plasmid

   #171145

   Purpose

   Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J109 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFETCh_NFIL3

   Plasmid

   #86287

   Purpose

   Donor vector for 3' FLAG tag of human NFIL3

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   NFIL3 homology arms (NFIL3 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK2013-AkCas12b

   Plasmid

   #121950

   Purpose

   E. coli expression, protein purification

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AkCas12b
   

   Tags

   10xHis tag and 3xHA tag

   Expression

   Bacterial

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid

    #133340

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

    Plasmid

    #191360

    Purpose

    Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dHgm4Cas13b-EGFP-NLS
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas12j3 KRAB

    Plasmid

    #188507

    Purpose

    Expresses FLAG tagged dCas12j3 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-3a-His-MBP-TEV-VC_Cas1_WT

    Plasmid

    #120364

    Purpose

    Expresses wildtype V-C Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VC_Cas1
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-3a-His-MBP-TEV-VD_Cas1_WT

    Plasmid

    #120365

    Purpose

    Expresses wildtype V-D Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

    Insert

    VD_Cas1
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pM116: CasRx VEGFA presgRNA

    Plasmid

    #166868

    Purpose

    U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting presgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFETCh_MIXL1

    Plasmid

    #86280

    Purpose

    Donor vector for 3' FLAG tag of human MIXL1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    MIXL1 homology arms (MIXL1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFETCh_TEAD3

    Plasmid

    #86281

    Purpose

    Donor vector for 3' FLAG tag of human TEAD3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    TEAD3 homology arms (TEAD3 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL11023

    Plasmid

    #117542

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
    

    Expression

    Plant

    Promoter

    2x35S_OMEGA(pICH51288)

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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