We narrowed to 30,677 results for: REP

1. 6xHis(TEV)-Drp1-StrepII

   Plasmid

   #174422

   Purpose

   Expresses human Drp1 Isoform 3 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-T7-Nla-repeat-BbsI

   Plasmid

   #180215

   Purpose

   For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-CAG_3C-Twin-Strep

   Plasmid

   #113901

   Purpose

   Mammalian protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX380 Treponema denticola Cas9

   Plasmid

   #68317

   Purpose

   Treponema denticola Cas9

   Depositor

   Feng Zhang

   Insert

   TdCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPuro3.1(+)_Strep-Lrrk2-Myc

   Plasmid

   #161583

   Purpose

   expression of full-length mouse Lrrk2 with C-terminal Myc tag

   Depositor

   Maik Hintze

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Use

   Tags

   Myc tag and StrepII

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. eGFP Y93H TC editing reporter

   Plasmid

   #198882

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Y93H

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-K9 histone methylation reporter

   Plasmid

   #22866

   Depositor

   Alice Ting

   Article

   Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

   Insert

   K9 HMR
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-StrepHA-SUMO2(Q87R)

   Plasmid

   #66868

   Purpose

   Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

   Depositor

   Ying Liu

   Article

   Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA and Strep

   Expression

   Mammalian

   Mutation

   Q87R

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastbac-Dual-StrepII-BARD1

   Plasmid

   #137166

   Purpose

   Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   BARD1 (BARD1 Human)
   

   Use

   Tags

   StrepII

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   p10

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. miRFP718nano-based inflammation reporter

    Plasmid

    #195753

    Purpose

    Reporter of inflamation

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    NF-kB-RE-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC414 - pAAV EF1a hPREP

    Plasmid

    #59967

    Purpose

    An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    Prolyl oligopeptidase (PREP Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRepair-MPP8_mAID-P2A-BFP

    Plasmid

    #175036

    Purpose

    Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    mAID-P2A-BFP
    

    Use

    Hdr donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIN REP5-GFP-GluR2 (Q)

    Plasmid

    #24003

    Depositor

    Robert Malinow

    Article

    Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

    Insert

    GluR2 (Gria2 Rat)
    

    Use

    Sindbis virus production

    Tags

    GFP

    Expression

    Mutation

    R607Q

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-2xFLAG-2xSTREP-CCND1

    Plasmid

    #172648

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-REPORT(PGK)-12xHRE

    Plasmid

    #172334

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHS0663 pET45b(+) KraIscB-1 TwinStrep

    Plasmid

    #176536

    Purpose

    Bacterial expression of KraIscB-1-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    KraIscB-1
    

    Use

    Tags

    TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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