We narrowed to 13,616 results for: ef1a;

1. c-myc-PT3EF1a

   Plasmid

   #92046

   Purpose

   Expresses c-Myc in mammalian cells

   Depositor

   Xin Chen

   Article

   Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

   Insert

   C-MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   July 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1aH

   Plasmid

   #180149

   Purpose

   This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

   Depositor

   Xin Chen

   Article

   Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-EF1a-TetOn3G

   Plasmid

   #104543

   Purpose

   PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   Tet-On 3G
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p274 EF1a_EGFP_hH11

   Plasmid

   #164851

   Purpose

   EGFP-expression vector for human H11 locus

   Depositor

   Philip Jordan

   Article

   Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDV05.0_pEF1a-iRFP720

   Plasmid

   #200407

   Purpose

   Expresses iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.EF1a.ChR2-YFP.WPRE.hGH

   Plasmid

   #100056

   Purpose

   AAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Insert

   ChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_TRE_sg30-PDGFR_EF1a_mCherry

   Plasmid

   #186309

   Purpose

   Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg30-PDGFR
   mCherry
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. RABGEF1A-c014

   Plasmid

   #175478

   Purpose

   Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   RABGEF1 (RABGEF1 Human)
   

   Use

   Tags

   His6-Zbasic-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDV05.2_pEF1a-iRFP720_secreted

   Plasmid

   #200409

   Purpose

   Expresses a secreted version of iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720 (secreted)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_EF1a_Flag_TP53_R248W+P278A

    Plasmid

    #183115

    Purpose

    Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 278 to alanine and arginine 248 t…

    Promoter

    EF1a

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid

    #186310

    Purpose

    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg61-PDGFR
    eGFP
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_Ef1a-->flagLaM4_Notch_tTA

    Plasmid

    #162237

    Purpose

    Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaM4 mCherry nanobody Notch tTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-mRor2WT

    Plasmid

    #22613

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

    Insert

    Ror2 WT (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a_MYC deltaCTD_P2A_Hygro_Barcode

    Plasmid

    #120511

    Purpose

    Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaCTD (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of carboxy-terminal domain

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_MYC deltaNTD_P2A_Hygro_Barcode

    Plasmid

    #120510

    Purpose

    Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaNTD (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of amino-terminal domain

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_MYC deltaMBI_P2A_Hygro_Barcode

    Plasmid

    #120504

    Purpose

    Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaMBI (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of MYC Box I domain

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a, U6

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_MYC deltab_P2A_Hygro_Barcode

    Plasmid

    #120507

    Purpose

    Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltab (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of basic motif

    Promoter

    EF1a

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

    Plasmid

    #120505

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    c-MYC deltaMBII (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of MYC Box II domain

    Promoter

    EF1a

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV-EF1aGFP2AKAT7-W

    Plasmid

    #159292

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 (KAT7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10)

    Promoter

    human EF1a promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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