We narrowed to 21,114 results for: NSI

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pTRIP-CMV-puro-2A-BLM

Plasmid

#127641

Purpose

Over-expression of human BLM

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

BLM (BLM Human)

Use

Lentiviral

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Epac-S-H81

Plasmid

#170339

Purpose

GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

Insert

GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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UPB-esophagus (UPB5)

Plasmid

#180783

Purpose

Unique Projection Barcode (UPB5)-Esophagus for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM3Dq ( partial ) (CHRM3 Human)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a-Nlux

Plasmid

#176239

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Promoter

Ribosomal subunit

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLL8 lenti-D10Acas9-3xflag-blast

Plasmid

#112134

Purpose

lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

D10Acas9-3xflag-blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A mutant in Cas9

Promoter

EF1a

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2-1/2

Plasmid

#91480

Purpose

Protein expression and purification of human SH3 domain construct GRB2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-1/2 (GRB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_BTK-1/1

Plasmid

#91350

Purpose

Protein expression and purification of human SH3 domain construct BTK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BTK-1/1 (BTK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgCIC-2

Plasmid

#74959

Purpose

Cas9 + sgCIC-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

CIC (CIC Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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Nsp10-mCherry

Plasmid

#165135

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp10 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(WT)-mCherry

Plasmid

#79421

Purpose

Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Nsp7-EGFP

Plasmid

#165111

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mScarlet

Plasmid

#169218

Purpose

Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB2 200bmut

Plasmid

#35538

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB2 gene (ZEB2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

6 Mutations in the miR-200b seed regions of the Z…

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pHES795

Plasmid

#87943

Purpose

Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

Zif268 DBD - hER LBD - MSN2 AD (ESR1 Human, Synthetic, Budding Yeast)

Expression

Yeast

Promoter

ADH1 (crippled)

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_BCAR1-1/1

Plasmid

#91418

Purpose

Protein expression and purification of human SH3 domain construct BCAR1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BCAR1-1/1 (BCAR1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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HR-TERT-SV40-GFP

Plasmid

#71397

Purpose

Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Tags

SV-40 GFP insertion in TERT promotor

Promoter

Endogenous TERT promotor

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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OA-1136J

Plasmid

#153023

Purpose

Express His-MBP-TEV-CasRx recombinant protein

Depositor

Omar Akbari

Article

Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

Insert

CasRx

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITK-1/1

Plasmid

#91253

Purpose

Protein expression and purification of human SH3 domain construct ITK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITK-1/1 (ITK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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