We narrowed to 15,699 results for: NOL

1. pJBL705

   Plasmid

   #140375

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-5BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ_lacO-cP-AOX1-CPR

   Plasmid

   #126742

   Purpose

   yeast plasmid overexpressing CPR

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   cpr
   

   Tags

   6xHis

   Expression

   Yeast

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. IBMc071

   Plasmid

   #161943

   Purpose

   Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNAS1b split mCherry 14-3-3σ

   Plasmid

   #111881

   Purpose

   Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Tags

   Split mCherry system: 14-3-3σ fused to C-terminal…

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAL2294

   Plasmid

   #36727

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   APC-TALEN_Left (APC Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. 1161F

   Plasmid

   #183138

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 HDAC1

   Plasmid

   #67101

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCfB3050(gRNA XII-5)

   Plasmid

   #73292

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX Aps-SH2

    Plasmid

    #46404

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Aps (SH2B2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 404-523

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC121

    Plasmid

    #118893

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and MBP, cleavab…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CP1041-ABE8e-dimer

    Plasmid

    #138499

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TAL2272

    Plasmid

    #36709

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    KRAS-TALEN_Left (Kras Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. Zebrafish-gRNA-0008

    Plasmid

    #42248

    Purpose

    gRNA targeted to zebrafish gene tia1l

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-tia1l (tia1l Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

15. TAL2273

    Plasmid

    #36710

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    KRAS-TALEN_Right (Kras Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pLVX-Tight-Puro_Myt1

    Plasmid

    #64845

    Purpose

    A lentiviral vector for the expression of mouse Myt1 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Myt1 (Myt1 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB526

    Plasmid

    #74868

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. SaKKH-ABE8e-dimer

    Plasmid

    #138503

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPD2 AtUBQ10 (GB2478)

    Plasmid

    #160594

    Purpose

    UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtUBQ10
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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