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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,447 results for: grn

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Showing: 7501 - 7520 of 15447 results

  1. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Publication
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  6. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  11. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Publication
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  13. pJP_Osc05

    Plasmid
    #229040
    Purpose
    Light inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.
    Depositor
    Yolanda Schaerli
    Publication
    Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038
    Insert
    pBAD promoter
    Expression
    Bacterial
    Available since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229040-plasmid-map-sequence-id-458369

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  15. pMA-SpCas9-g5

    Plasmid
    #80788
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80788-plasmid-map-sequence-id-162408

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  16. pX330-PITCh-LMNB1

    Plasmid
    #207770
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207770-plasmid-map-sequence-id-416199

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Showing: 7501 - 7520 of 15447 results