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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1.2

Plasmid

#113937

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1.2

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1C3-H3

Plasmid

#109381

Purpose

Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1C3-H3

Plasmid

#109381

Purpose

Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt2

Plasmid

#64832

Purpose

Lentiviral expression of murine Flag-Akt2

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt2 (Akt2 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-IRES-tdTomato-FlagAkt2

Plasmid

#64832

Purpose

Lentiviral expression of murine Flag-Akt2

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt2 (Akt2 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pME-mKate2-NS

Plasmid

#109729

Purpose

Multisite gateway vector for 5' tagging with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-NS

Plasmid

#109729

Purpose

Multisite gateway vector for 5' tagging with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-NS

Plasmid

#75341

Purpose

Multisite gateway entry clone for adding N-terminal fusions of EGFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-NS

Plasmid

#75341

Purpose

Multisite gateway entry clone for adding N-terminal fusions of EGFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-APEX2-NS

Plasmid

#108895

Purpose

Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2

Use

Multisite gateway

Available Since

May 19, 2018

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-APEX2-NS

Plasmid

#108895

Purpose

Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2

Use

Multisite gateway

Available Since

May 19, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-mVenus

Plasmid

#67719

Purpose

Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

mVenus

Use

Gateway multisite 3' entry clone

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-mVenus

Plasmid

#67719

Purpose

Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

mVenus

Use

Gateway multisite 3' entry clone

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60626

Purpose

Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP16 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60626

Purpose

Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP16 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge