We narrowed to 23,367 results for: Nov;

1. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

   Plasmid

   #127177

   Purpose

   N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   Flag- HA- Tandem Epitopes

   Expression

   Mutation

   Promoter

   None

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-LaM-8

   Plasmid

   #172772

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-8
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pF709-pET28a-Hs-FL-ETFbeta-NHis wt

   Plasmid

   #85110

   Purpose

   expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length ETFbeta (ETFB Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-HA-TRAIL-R1

   Plasmid

   #61382

   Purpose

   Expresses HA tagged TRAIL receptor 1

   Depositor

   Francis Chan

   Article

   Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

   Insert

   TRAIL receptor 1
   

   Use

   Tags

   HA and TNF receptor leader peptide

   Expression

   Mammalian

   Mutation

   TRAIL-R1 leader peptide (aa1-23) replaced with TN…

   Promoter

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PB-Neo-TetO-Cdk12_Untagged

   Plasmid

   #127178

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Piggybac transposase vector

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   TetO Promoter

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-MCPIP1 (1-599)

   Plasmid

   #127461

   Purpose

   Expressing Flag-tagged human MCPIP1 full lengh protein in mammalian cells

   Depositor

   Mingui Fu

   Article

   Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

   Insert

   MCPIP1 (ZC3H12A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

   Plasmid

   #139448

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-LaG-41-4GS-LaG-2

   Plasmid

   #172766

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41-4GS-LaG-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCIG PIK3CA Wildtype

   Plasmid

   #73056

   Purpose

   Human PIK3CA Wildtype

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   PIK3CA Wildtype (PIK3CA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-hLC3

    Plasmid

    #87872

    Purpose

    Express a EGFP-human MAP1-LC3 in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    MAP1LC3 (MAP1LC3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

    Plasmid

    #197066

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    CRISPR

    Tags

    FKBP and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaG-10

    Plasmid

    #172743

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-10
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG-18

    Plasmid

    #172763

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-18
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaG-42

    Plasmid

    #172758

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-42
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaG-16-3xFLAG-LaG-2

    Plasmid

    #172765

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-3xFLAG-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HsTPC2-EYFP

    Plasmid

    #135194

    Purpose

    Expresses tagged TPC2 in mammalian cells

    Depositor

    Antony Galione

    Article

    Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

    Insert

    TPC2 (TPCN2 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaG-6

    Plasmid

    #172741

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-ms-Sox18

    Plasmid

    #50782

    Purpose

    retroviral expression of mouse Sox18

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox18 (Sox18 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDK637

    Plasmid

    #134203

    Purpose

    bacterial expression of human CENP-N 1-240

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-N 1-240-His (CENPN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178574

    Purpose

    Lentiviral vector for CamKIIα::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21-pelB-LaG-21

    Plasmid

    #172749

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-21
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LentiGuidPuro-hTP63_sgRNA-1

    Plasmid

    #88854

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-mPCSK8

    Plasmid

    #122677

    Purpose

    Expresses mouse PCSK8 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CaMKII-soCoChR-GFP

    Plasmid

    #107711

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

    Plasmid

    #178575

    Purpose

    Lentiviral vector for GFAP::Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CRY2PHR-P2A-CIBN-mCherry-VAMP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GFAP

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21-pelB-LaG-41

    Plasmid

    #172757

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. H6SUMO-IntC-P53(304-393)

    Plasmid

    #200316

    Purpose

    bacterial expression of P53 TET-CTD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (304-393) (TP53 Human)
    

    Use

    Tags

    His6-SUMO and designed intein C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge