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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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161,472 results

Showing: 11671 - 11700 of 161472 results

  1. Chromoprotein kit 2.0

    Plasmid Kit
    #1000000198
    Purpose
    Chromoprotein plasmid kit that contain two new chromoprotein genes that are less toxic to E. coli so that the plasmids are more stable during growth.
    Depositor
    Anthony Forster
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only

  3. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

    Plasmid
    #164615
    Purpose
    Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    Renilla luciferase
    Use
    Tags
    24xMS2v5 (3'UTR) and SunTag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164615-plasmid-map-sequence-id-325006

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  5. GPX4

    Plasmid
    #38797
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX4 (GPX4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys73 -> Gly
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38797-plasmid-map-sequence-id-428950

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  6. EcSTH-LbNOX

    Plasmid
    #211924
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.
    Depositor
    Binghui Li
    Article
    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:
    Insert
    EcSTH-LbNOX
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    211924-plasmid-map-sequence-id-419891

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  11. pGES401

    Plasmid
    #190198
    Purpose
    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection
    Depositor
    Yuefeng Guan
    Article
    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190198-plasmid-map-sequence-id-371936

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  17. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

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  18. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

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  20. pM1s3TsR

    Plasmid
    #137922
    Purpose
    E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    137922-plasmid-map-sequence-id-323851

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  25. pAAV-Ef1a-Con/Foff 2.0-oScarlet (AAV8)

    Viral Prep
    #137137-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-oScarlet (#137137). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-oScarlet plasmid DNA. EF1a-driven, Cre-dependent expression of oScarlet (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    oScarlet (Cre-dependent)
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  29. Tet-pLKO-FOXM1-shRNA-84

    Plasmid
    #89496
    Purpose
    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    Aug. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    89496-plasmid-map-sequence-id-190853

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Showing: 11671 - 11700 of 161472 results