165,586 results
-
Plasmid#225609PurposeSuicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sitesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only
-
pEND-224_Cacnes_sfGFP
Plasmid#225611PurposeReplicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.DepositorInsertsfGFP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_Txe/Axe
Plasmid#229147PurposeToxin-Antitoxin (Txe/Axe)DepositorInsertmCherry, Txe/Axe
UseTagsExpressionBacterialMutationPromoterPtlpA (mCherry), Txe/Axe (Native)Available sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-2
Plasmid#228984PurposeFor bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-2
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-1--LaG94-14
Plasmid#228973PurposeFor bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaG94-1--LaG94-14
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-1
Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-1
Plasmid#228974PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaTdT-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-7
Plasmid#228997PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-7
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-1
Plasmid#228995PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-5
Plasmid#228991PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-5
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCit(P5f-P13f)3-gs
Plasmid#228080PurposeMammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, P5f, P13f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCD-TVMVP
Plasmid#226226PurposeExpress TVMVPDepositorInsertTVMVP (TVMVgp1 Tobacco vein mottling virus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCR4-beta-globin::lacZ-H11
Plasmid#227000PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterbeta-globinAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(P6f)3(P14f)3-gs
Plasmid#228081PurposeMammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, P6f, P14f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hCAR.Variant3
Plasmid#209133PurposeThe ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.DepositorInsertconstitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 MERS-CoV NSP6_stop
Plasmid#168633PurposeGateway-compatible Entry vectorDepositorInsertNSP6 (orf1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 MERS-CoV NSP3_nostop
Plasmid#168608PurposeGateway-compatible Entry vectorDepositorInsertNSP3 (orf1ab )
UseOtherTagsExpressionMutationPromoterAvailable sinceJan. 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Kv1.2 K+ channel scFv [K14/16]
Plasmid#182064PurposeMammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.DepositorInsertrecombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESN2_GFP_YTA_Champ1_328-354
Plasmid#105639PurposeExpresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFPDepositorInsertChamp1 (CHAMP1 Human)
UseTagsGFP YTAExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_5a7_pKD46.10
Plasmid#178937PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAG-EnRRbpj
Plasmid#160925Purposedominant-negative construct to inhibit Notch signalingDepositorInsertEnRRbpj
UseTagsExpressionMutationfused with an engrailed repressorPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-mRIPK1-S321A
Plasmid#115339PurposeMammalian expression of Flag-mRIPK1-S321ADepositorInsertRIPK1 (Ripk1 Mouse)
UseTagsFlagExpressionMammalianMutationS321APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-Lifeact-7
Plasmid#54696PurposeLocalization: Actin, Excitation: 507 / 572, Emission: 516 / 580DepositorTypeEmpty backboneUseTagsmEos3.2-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF2528
Plasmid#144127PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertERCC6 (ERCC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERP2
Plasmid#80478PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100853PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV Retrograde and AAV1InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV5)
Viral Prep#105547-AAV5PurposeReady-to-use AAV5 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEGFPAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
HRE-oUnaG
Plasmid#201712PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1aDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only