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pAc5.1B-lambdaN-HA-DmAGO1-Y749A-siRNAres_F

Plasmid

#146341

Purpose

Insect Expression of DmAGO1-Y749A-siRNAres

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmAGO1-Y749A-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmAGO1-PAZ6mut-siRNAres_F

Plasmid

#146319

Purpose

Insect Expression of DmAGO1-PAZ6mut-siRNAres

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmAGO1-PAZ6mut-siRNAres (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GLP1R-NTEV-TCS-GV-2xHA

Plasmid

#194379

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GLP1R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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JH204: pMVP (R4-R3) human PDX1 open

Plasmid

#121745

Purpose

pMVP R4-R3 entry plasmid, contains human PDX1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Human PDX1 (open) (PDX1 Human)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

Plasmid

#83458

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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exon K-renilla

Plasmid

#81007

Purpose

luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

Depositor

Richard Baines

Article

Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

Insert

paralytic (para Fly)

Tags

Renilla luciferase

Expression

Insect

Mutation

spanning exon 24 to exon 26, termination codon in…

Promoter

P-AC5 (Actin 5C)

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-Delta-ATG1-mMCL1

Plasmid

#45823

Purpose

This plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

Delta ATG1-mMCL1

Expression

Mammalian

Mutation

The first amino acid (ATG) is deleted from the fu…

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-N-Drd1

Plasmid

#104358

Purpose

Expresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.

Depositor

Kirk Mykytyn

Article

Domire et al Cell Mol Life Sci. 2011 Sep;68(17):2951-

Insert

Dopamine receptor D1 (Drd1) (Drd1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.27_ARE/NRF2-SPE

Plasmid

#177775

Purpose

Luciferase reporter for ARE/NRF2

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

NRF2 (NFE2L2 Human)

Use

Luciferase

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mHif1a

Plasmid

#242809

Purpose

Lentiviral vector expressing mouse Hif1a tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

Hypoxia-inducible factor 1, alpha subunit (Hif1a Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SMS1-TEV-HAT

Plasmid

#202557

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

SGMS1 (SGMS1 Human)

Tags

Histidine-Affinity-tag (HAT) and TEV cleavable si…

Expression

Mammalian

Mutation

deleted stop codon (TAA)

Promoter

CAG and chicken β-actin promoter

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A8

Plasmid

#31114

Depositor

Gerhart Ryffel

Article

Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

Insert

HNF4A (HNF4A Human)

Tags

Myc

Expression

Mammalian

Mutation

splice variant 8

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-Zc3h12a 3’UTR

Plasmid

#222662

Purpose

Luciferase reporter vector containing mouse Zc3h12a 3'UTR

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a 3'UTR (Zc3h12a Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-ddMSK1

Plasmid

#165603

Purpose

Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Synthetic, Human, S. Pyogenes)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

EF1a

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

Plasmid

#202822

Purpose

Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

two sgRNAs that delete exon 1 within the ZNF91 gene

Use

Lentiviral

Expression

Mammalian

Promoter

U6 - two

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-PHLPP1 full length

Plasmid

#37100

Purpose

Expresses HA tagged, full-length human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

Insert

PHLPP1 (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHes1(467)-luc

Plasmid

#41723

Depositor

Ryoichiro Kageyama, Raphael Kopan

Article

Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

Insert

Hes1 Promoter (-467 to +46) (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter (-467 to +46)

Promoter

Hes1 promoter fragment (-467 to +46)

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194381

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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