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Showing: 121 - 160 of 22446 results

  1. UbiGate E2 activity screen (set2)

    Plasmid Kit
    #1000000145
    Purpose
    Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74707-plasmid-map-sequence-id-152737

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  10. pLKO.1C-YFP-shSCR

    Plasmid
    #139647
    Purpose
    Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.
    Depositor
    David Cobrinik
    Article
    Lee et al Biotechniques. 2020 Jun;68(6):349-352. d
    Insert
    shSCR
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor comments
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    139647-plasmid-map-sequence-id-337632

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  14. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid
    #74705
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74705-plasmid-map-sequence-id-151422

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  18. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid
    #74709
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. pBluescript mouse ptc

    Plasmid
    #58699
    Purpose
    full length mouse ptc cDNA
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pBluescript alpha-SSeCKS

    Plasmid
    #61295
    Purpose
    contains rat alpha SSeCKS cDNA
    Depositor
    Irwin Gelman
    Insert
    alpha SSeCKS (Akap12 Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cDNA with 1kb of 3' UTR and 150bp of 5'…
    Promoter
    T7
    Available since
    Dec. 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. R777-E305 Hs.SCRIB

    Plasmid
    #70589
    Purpose
    Gateway ORF clone of human SCRIB [NM_182706.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SCRIB (SCRIB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70589-plasmid-map-sequence-id-133940

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  39. LLhScrR-pHG165a 3mut

    Plasmid
    #220278
    Purpose
    Constitutive expression of a chimeric LacI:ScrR transcription repressor
    Depositor
    Liskin Swint-Kruse
    Insert
    LLhScrR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V52I/Q62S/G120S
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    220278-plasmid-map-sequence-id-436472

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Showing: 121 - 160 of 22446 results