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pLCKO2::TKOv3

Pooled Library

#125517

Purpose

Human CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backbone

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only
Liu Lab MusCK Library A

Pooled Library

#174196

Purpose

MusCK library contains 23,622 unique sgRNAs targeting 4,787 gene locations in the genome. Sub-library MusCK A contains 5 gRNAs per gene.

Depositor

Xiaole Shirley Liu

Article

Wang et al Cell. 2021 Oct 14;184(21):5357-5374.e22.

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only
Human Base Editing Sensor Library

Pooled Library

#179218

Purpose

sgRNAs that target frequently occurring cancer associated mutations identified in the MSK IMPACT dataset that can be modeled with cytosine base editing.

Depositor

Lukas Dow

Article

Sanchez-Rivera et al Nat Biotechnol. 2022 Feb 14. pii: 10.103

Expression

Mammalian

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only
Mouse Base Editing Sensor Library

Pooled Library

#179217

Purpose

sgRNAs that target frequently occurring cancer associated mutations identified in the MSK IMPACT dataset that can be modeled with cytosine base editing.

Depositor

Lukas Dow

Article

Sanchez-Rivera et al Nat Biotechnol. 2022 Feb 14. pii: 10.103

Expression

Mammalian

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only
pCVSN2c-MCP-oScarlet-KASH-6xMS2 Barcode Library

Pooled Library

#227672

Purpose

RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of CVS N2c strain G-deleted rabies virus

Depositor

Cathryn Cadwell, Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Expression

Mammalian

Species

Synthetic

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only
Liu Lab MusCK Library B

Pooled Library

#174197

Purpose

MusCK library contains 23,622 unique sgRNAs targeting 4,787 gene locations in the genome. Sub-library MusCK M_b contains 5 gRNAs per gene.

Depositor

Xiaole Shirley Liu

Article

Wang et al Cell. 2021 Oct 14;184(21):5357-5374.e22.

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only
CHyMErA Large-Scale Exon Deletion hgRNA Library

Pooled Library

#209736

Purpose

CHyMErA Exon Deletion hgRNA Library allows identification of frame-preserving exons that affect cell fitness in human cells using the CHyMErA screening platform.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only
HR700_TP53Exon5mut

Pooled Library

#229137

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 5 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
HR700_TP53Exon6mut

Pooled Library

#229138

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 6 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
HR700_TP53Exon7mut

Pooled Library

#229139

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 7 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
HR700_TP53Exon8mut

Pooled Library

#229140

Purpose

Donor vector library to introduce TP53 mutations into the endogenous gene locus at exon 8 of human cells via CRISPR-HDR.

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Cre/Lox

Available since

Feb. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits
ALL-PAM sensor Library

Pooled Library

#179219

Purpose

10 human and mouse sgRNAs each where for each sgRNA the tethered target site has 1 of 64 possible 3 bp PAM sequences.

Depositor

Lukas Dow

Article

Sanchez-Rivera et al Nat Biotechnol. 2022 Feb 14. pii: 10.103

Expression

Mammalian

Use

Lentiviral

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only
Barcode Library 0 (BCL0)

Pooled Library

#179851

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code ACGT.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
Barcode Library 1 (BCL1)

Pooled Library

#179852

Purpose

Lentiviral barcode library that consists of 10^6 unique barcodes. Barcodes consist of 12bp degenerate region followed by two stable bases (C or G) and 4bp unique library code CCAA.

Depositor

Jason Moffat

Article

Rehman et al Cell. 2021 Jan 7;184(1):226-242.e21. doi

Expression

Mammalian

Use

Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only
CROPseq-iT7 Human CRISPR Knockout Pooled Library (Brunello)

Pooled Library

#223064

Purpose

Optical pooled genome-scale CRISPR KO screening using Nuclear In-Situ Sequencing (NIS-Seq)

Depositor

Jonathan Schmid-Burgk

Article

Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 23, 2025

Availability

Academic Institutions and Nonprofits only
Green monkey (Chlorocebus sabaeus) sgRNA library

Pooled Library

#178284

Purpose

Genome-wide CRISPR screening library targeting 20,991 genes in C. sabaeus, 84,963 total gRNAs

Depositor

David Root, John Doench

Article

Wei et al Cell. 2021 Jan 7;184(1):76-91.e13. doi:

Expression

Mammalian

Species

Chlorocebus sabaeus (african green monkey)

Use

CRISPR and Lentiviral

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only
Mouse Validation (mVAL) CRISPR Library

Pooled Library

#159391

Purpose

Pooled CRISPR library targets core intrinsic immune evasion genes

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2

Pooled Library

#159392

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - pLCKO2

Pooled Library

#159393

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
SmoCFP

Plasmid

#128156

Purpose

Expression of Smoothened

Depositor

Jennifer Lippincott-Schwartz

Article

Ott et al Cilia. 2012 Apr 25;1(1):3. doi: 10.1186/

Insert

Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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f-CTS-EGFP

Plasmid

#86064

Purpose

A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains ciliary targeting signal (3870-3887 amin…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e

Plasmid

#193645

Purpose

Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y132D

Plasmid

#193641

Purpose

Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y132D

Use

Tags

Expression

Mammalian

Mutation

hAPOBEC3A-Y132D

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCas12a-A3B

Plasmid

#193642

Purpose

Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3B, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCas12a-A3G

Plasmid

#193643

Purpose

Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3G, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CD8a-f-CTS-EGFP

Plasmid

#86063

Purpose

A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains CD8a luminal and transmembrane domains a…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCas12a-A3A

Plasmid

#193638

Purpose

Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCas12a-AID

Plasmid

#193647

Purpose

Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hAID, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCas12a-BE

Plasmid

#193648

Purpose

Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-rAPOBEC1, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCas12a-CDA1

Plasmid

#193649

Purpose

Expression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-PmCDA1, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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