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  1. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet (AAV8)

    Viral Prep
    #137161-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet (#137161). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet plasmid DNA. nEF-driven expression of ChRmine-oScarlet in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    oScarlet (Cre-dependent)
    Available Since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  11. pAAV.GfaABC1D.PI.Lck-GFP.SV40 (AAV5)

    Viral Prep
    #105598-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA. GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Shigetomi et al J Gen Physiol. 2013 Apr 15.
    Promoter
    GfaABC1D
    Tags
    GFP
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pRSETB/mStayGold

    Plasmid
    #212017
    Purpose
    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Type
    Empty backbone
    Tags
    6xHis, T7 tag, Xpress tag, mStayGold
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212017-plasmid-map-sequence-id-419228

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