175,888 results

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EF.GFP

Plasmid

#17616

Depositor

Linzhao Cheng

Article

Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Insert

EF-1a promoter (EEF1A1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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HEK4-53bp-tag-insertion-pegRNA

Plasmid

#227673

Purpose

PegRNA for 53 bp insertion

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

SpCas9 sgRNA scaffold (EPHA3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV LIC cloning vector (2S-T)

Plasmid

#29711

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, SUMO, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pCIpA102-G-HLA-A2_GFP

Plasmid

#85162

Purpose

for mRNA expression of HLA.A2-EGFP

Depositor

Tone Fredsvik Gregers, Sébastien Walchli

Article

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Mrna expression

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLV6-Bmal-luc

Plasmid

#68833

Purpose

Expression of Bmal1-luc in mammalian cells

Depositor

Steven Brown

Insert

Bmal1 regulatory sequences (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-H2B-PAmCherry

Plasmid

#133419

Purpose

Fluorescent reporter for calcium imaging and photoactivation

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-GFP1-10

Plasmid

#80409

Purpose

Expresses GFP1-10 in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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TFORF1525

Plasmid

#143515

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GFI1 (GFI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-3xHA

Plasmid

#207685

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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LAMP1-SEP-mRuby3

Plasmid

#200939

Purpose

endolysosome localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

LAMP1 (LAMP1 Human)
SEP-mRuby3

Use

Lentiviral

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-Furin

Plasmid

#179920

Purpose

Furin protein

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Furin

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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mTagRFP-Membrane-1

Plasmid

#57992

Purpose

Localization: Plasma Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Type

Empty backbone

Tags

N-terminal 20 Aas MLS from Neuromodulin and mTagR…

Expression

Mammalian

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCLX-PGK-hCDK4

Plasmid

#114314

Purpose

Constitutive expression of the CDK4 protein

Depositor

Patrick Salmon

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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