165,545 results

1. pLV hUbC-Cas9-T2A-GFP

   Plasmid

   #53190

   Purpose

   3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-Nsp16

   Plasmid

   #157723

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGRG25

   Plasmid

   #16665

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

   Insert

   mTn7::MCS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM-41-HsPABPC1_J

   Plasmid

   #146644

   Purpose

   Bacterial Expression of HsPABPc1

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   HsPABPc1 (PABPC1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations compared to the sequence giv…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HP17deltaP8

   Plasmid

   #235447

   Purpose

   Helper plasmid for regulated phage transfer

   Depositor

   Yolanda Schaerli

   Article

   et al Nat Commun 16, 3569 (2025)

   Insert

   Coding part of the M13KO7 helper phage genome, except gene VIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PMXS-SLC1A3

   Plasmid

   #72873

   Purpose

   The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   SLC1A3 (SLC1A3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. hSyn-DIO-somBiPOLES-mCerulean (AAV5)

   Viral Prep

   #154951-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Promoter

   Syn

   Tags

   mCerulean (Cre-dependent)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pRRL-MHCK7-GCaMP6

   Plasmid

   #65042

   Purpose

   Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

   Depositor

   Nenad Bursac

   Article

   Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

   Insert

   GCaMP6
   

   Use

   Lentiviral

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   MHCK7

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTNT-B18R

   Plasmid

   #58978

   Purpose

   expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   B18R
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Sp6

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3xFLAG-TYR

    Plasmid

    #32780

    Depositor

    Ruth Halaban

    Article

    Halaban et al J Invest Dermatol. 2002 Aug;119(2):481-8

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

12. mPlum-N1

    Plasmid

    #54629

    Purpose

    Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrichoGate-16

    Plasmid

    #160716

    Purpose

    T-nos Terminator bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    nopaline synthase terminator (T-nos) bordered by BsaI restriction sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Incorporation of BsaI restriction sites

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL103-SpCas9-FLAG-3XNLS

    Plasmid

    #182032

    Purpose

    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

    Depositor

    Steven Lin

    Article

    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

    Insert

    Streptococcus pyogenes cas9 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    12X His tag, 3XNLS, FLAG, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. NM1-5S-tRNA-SgH

    Plasmid

    #128178

    Purpose

    Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5S rRNA-tRNA(Arg)

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. OPA1-isoform1

    Plasmid

    #70173

    Purpose

    Mammalian expression of hsOPA1 isoform 1

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 1 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ782 - HygroR

    Plasmid

    #190933

    Purpose

    Hygromycin resistance co-injection marker

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Hygromycin resistance gene
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV3TAG8li-hSTT3A-3FLAG

    Plasmid

    #159140

    Purpose

    Hu STT3A -flag mammalian expression vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    STT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac1-6His-DHX9

    Plasmid

    #211440

    Purpose

    generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

    Depositor

    Helmut Pospiech, Frank Grosse

    Article

    Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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