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pNeonGreen-C1-Nir2-LNS2

Plasmid

#220086

Purpose

C-terminal PA binding domain of Nir2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM1 (PITPNM1 Human)

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 896-1216

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO1

Plasmid

#53138

Purpose

E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-97)

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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AiP11457 - pAAV-eHGT_078h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1457)

Plasmid

#163495

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304-KDM6A

Plasmid

#203580

Purpose

Expresses wild-type KDM6A in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

Insert

KDM6A (KDM6A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Parkin

Plasmid

#187897

Purpose

Mammalian cell expression of untagged Parkin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

PRKN (PRKN Human)

Tags

None

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

hrGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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1436 pcDNA3 Flag HA

Plasmid

#10792

Purpose

pcDNA3 vector with added Flag, HA tags

Depositor

William Sellers

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

FLAG and HA

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

Plasmid

#174081

Purpose

Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

Insert

NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0

Tags

NES-Flag

Expression

Mammalian

Mutation

L270G, Y271L, N311G, C313A

Promoter

CMV

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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hMEF2A WT

Plasmid

#118354

Purpose

This plasmid expresses human MEF2A-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158741

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/MI/45/15 H1N1

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLIX403-EBFP2-ccdB-G418

Plasmid

#158556

Purpose

Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-FRCaMPi

Plasmid

#232838

Purpose

AAV transfer plasmid for Syn-mediated expression of FRCaMPi

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomaFRCaMPi (NES Synthetic)

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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LAMP1-Venus

Plasmid

#234750

Purpose

Mammalian expression of lysosome marker LAMP1-Venus

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

LAMP1-Venus

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Sec31a #861#640

Plasmid

#42123

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
pBad-LgBiT-PhoCl1-SmBiT-MBP

Plasmid

#164034

Purpose

luciferase-based PhoCl screening construct

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

LgBiT-PhoCl1-SmBiT-MBP

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-BFP-24xPP7

Plasmid

#74929

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-BFP-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

Plasmid

#207507

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pcDNA6)

Plasmid

#26578

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Tags

His6 and V5

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

Plasmid

#224501

Purpose

Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lamin B Vhh mNeonGreen-pA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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