167,785 results

1. pEGFP-rUSP19CYT

   Plasmid

   #155248

   Purpose

   for mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminus

   Depositor

   Simon Wing

   Insert

   USP19 (Cytoplasmic isoform) (Usp19 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-rUSP19ER

   Plasmid

   #155247

   Purpose

   for mammalian overexpression of the ER isoform of rat USP19 fused to GFP at N-terminus

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPEE16

   Plasmid

   #128360

   Purpose

   Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 1, a small gene-free region. Contains the amdS2 counterselectable marker.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Type

   Empty backbone

   Use

   Unspecified

   Promoter

   TEF1

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pre_IV_CS10_1033_v1

   Plasmid

   #126860

   Purpose

   4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1033_v1
   

   Use

   Synthetic Biology

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PKNOX1_pCSdest

   Plasmid

   #53899

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   PKNOX1 (PKNOX1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCR1265

   Plasmid

   #111094

   Purpose

   lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

   Plasmid

   #203350

   Purpose

   Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Christopher Grigsby, Molly Stevens

   Insert

   Nano Luciferase
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Promoter

   T7

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRi(v2)-Blast

   Plasmid

   #170068

   Purpose

   Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SIRT3 Flag

   Plasmid

   #13814

   Purpose

   Mammalian expression of human SIRT3 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT3 (SIRT3 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF5B-FRT-GFP-αTAT1[D157N]

    Plasmid

    #27100

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Tags

    GFP and S-tag

    Expression

    Mammalian

    Mutation

    D157N

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human SLC KO Library

    Pooled Library

    #132552

    Purpose

    Expresses gRNAs for knockout of the human Solute Carrier family

    Depositor

    Giulio Superti-Furga

    Article

    Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

12. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid

    #193298

    Purpose

    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-HTG

    Plasmid

    #27437

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

    Insert

    histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
    

    Use

    AAV; Adeno-associated viral

    Mutation

    N/A

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tandem p40p35

    Plasmid

    #108665

    Purpose

    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

    Depositor

    Nevil Singh

    Insert

    interleukin-12 (Il12a, Il12b Mouse)
    

    Expression

    Mammalian

    Mutation

    IL-12 dimers have a Gly-Ser linker

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Synapto-pHluorin

    Plasmid

    #168510

    Purpose

    Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    Synaptophysin-pHluorin (Syp Mouse)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAT7

    Plasmid

    #204292

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Promoter

    3XP3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-HisD-mNG-Ec-Kbp-D9N

    Plasmid

    #178011

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Ec-Kbp-D9N
    

    Expression

    Bacterial

    Promoter

    araB

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid

    #170378

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    human Synapsin promoter

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-GRP78-Wild-Type

    Plasmid

    #235169

    Purpose

    Expresses Wild-Type GRP78 in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Zhang et al J Biol Chem. 2010 May 14;285(20):15065-1

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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