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MSCV-N EBNA1

Plasmid

#37954

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBbS5a-Opto-Cre-Vvd-2

Plasmid

#160400

Purpose

Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd-2

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-EGFP-L

Plasmid

#52480

Purpose

Expresses mCherry and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-p53-shRNA-941

Plasmid

#25637

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-941 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pSJ55 Accessory plasmid with wt T7 DNAP

Plasmid

#253998

Purpose

Accessory plasmid for LySE, carrying wt T7 DNAP for propagation of phage T7∆DNAP

Depositor

Julius Fredens

Article

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

T7 DNAP (wt)

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_TOMM20-CLIP-tag2

Plasmid

#254210

Purpose

Mamalian cell expression of CLIP-tag2 on mitochondiral surface

Depositor

Kai Johnsson

Article

Johnsson et al bioRxiv 2026.03.05.709795

Insert

TOMM20-CLIP-tag2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pSJ139 Accessory plasmid with T7 DNAP v9

Plasmid

#254001

Purpose

Accessory plasmid for LySE, carrying error-prone T7 DNAP-TadDE (v9) for optimised mutational spectrum.

Depositor

Julius Fredens

Article

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

T7 DNAP v9

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4A-P2A-mCherry

Plasmid

#251135

Purpose

Gachapin-A with a transfection marker mCherry

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4A

Tags

P2A-mCherry

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-C2-P2A-mCherry

Plasmid

#251138

Purpose

Gachapin-C with a transfection marker mCherry

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-C2

Tags

P2A-mCherry

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4B-P2A-EBFP2

Plasmid

#251136

Purpose

Gachapin-B with a transfection marker EBFP2

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4B

Tags

P2A-EBFP2

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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TREM2-SmBiT Vector

Plasmid

#238801

Purpose

Express TREM2-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

TREM2 (TREM2 Human)

Tags

SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-STAG1 Vector

Plasmid

#238687

Purpose

Express NanoLuc-STAG1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

STAG1 (STAG1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_hSyn1_SypHer3s_mito

Plasmid

#238972

Purpose

Mammalian neurons SypHer3s expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

SypHer3s

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMJJ4-FOXO3-IRES-Thy1.1

Plasmid

#132703

Purpose

Expresses Thy1.1 and FOXO3

Depositor

Mandy Ford

Article

Krummey et al iScience. 2020 Apr 24;23(4):100912. doi:

Insert

FOXO3 (FOXO3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239020

Purpose

Co-overexpression of Rhobin2-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin2-H2B (H2BC21 Human, Synthetic)

Tags

TagBFP and mNeonGreen3K

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-EMSY (human)

Plasmid

#236420

Purpose

transient overexpression of EMSY in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

EMSY (EMSY Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2992

Plasmid

#144468

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXR2 (FOXR2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)

Plasmid

#233547

Purpose

A CMV driven human ZIC3 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

ZIC3 (ZIC3 Human)

Use

Lentiviral

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

Plasmid

#232131

Purpose

A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF2 (FOXF2 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Syn61

Bacterial Strain

#174513

Purpose

Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

Depositor

Jason W Chin

Article

Fredens et al Nature. 2019 May;569(7757):514-518. doi:

Bacterial Resistance

Streptomycin

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only