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Showing: 801 - 820 of 38516 results

  1. pGEX-2T-RNAPII-A2-14-HA

    Plasmid
    #160689
    Purpose
    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.
    Depositor
    Jeffry Corden
    Article
    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.
    Insert
    RNA polymerase II CTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Contains an S-to-A mutation in position 2 of 14 o…
    Promoter
    Available since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    160689-plasmid-map-sequence-id-317780

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  3. p3.2mar(T->G)-Luc

    Plasmid
    #63697
    Purpose
    3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expression
    Depositor
    David Kingsley
    Article
    O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755
    Insert
    3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
    Use
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    TATA box
    Available since
    May 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63697-plasmid-map-sequence-id-108763

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  4. p3.2mar(T->G)-GFP

    Plasmid
    #63695
    Purpose
    3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporter
    Depositor
    David Kingsley
    Article
    O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755
    Insert
    3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    T->G bp change
    Promoter
    hsp70
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63695-plasmid-map-sequence-id-106194

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  7. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid
    #192438
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    Use
    Tags
    Full-length LAMP1 and mCherry
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192438-plasmid-map-sequence-id-379134

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  10. ePop

    Plasmid
    #50953
    Purpose
    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations
    Depositor
    Lingchong You
    Article
    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:
    Inserts
    E lysis protein
    LuxI
    LuxR
    Use
    Tags
    Expression
    Mutation
    Early stop codon introduced in mutated luxR resul…
    Promoter
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50953-plasmid-map-sequence-id-454119

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  15. PF4-bio-His

    Plasmid
    #53411
    Purpose
    Expresses full-length Platelet factor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PF4 (PF4 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53411-plasmid-map-sequence-id-83194

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  19. MSCV-myrAkt

    Plasmid
    #49267
    Purpose
    retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
    Depositor
    Arthur Weiss, Lawrence Kane
    Article
    Kane et al Nat Immunol. 2001 Jan;2(1):37-44.
    Insert
    Akt (AKT1 Bovine)
    Use
    Retroviral
    Tags
    IRES-EGFP and myr
    Expression
    Mammalian
    Mutation
    N2D, D149E and A478G compared to AAW71957.1
    Promoter
    Available since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 820 of 38516 results