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We narrowed to 3,888 results for: dCas9

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Showing: 801 - 820 of 3888 results

  1. pLenti-mCherry-Cre-blast

    Plasmid
    #179390
    Purpose
    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    mCherry (P2A) Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179390-plasmid-map-sequence-id-451660

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  3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129027-plasmid-map-sequence-id-256129

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  6. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  14. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129025-plasmid-map-sequence-id-454365

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  15. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  16. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

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  19. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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Showing: 801 - 820 of 3888 results