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pNLF1-C_WDR12:1-423

Plasmid

#210953

Purpose

Mammalian expression of full-length human WDR12. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:M1-A423 (WDR12 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDR89:1-387

Plasmid

#211049

Purpose

Mammalian expression of full-length human WDR89. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR89:M1-Q387 (WDR89 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Mycn-IRES-GFP

Plasmid

#35394

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Mycn (Mycn Mouse)
GFP

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only
pSimpleII-NLS-NmCas9-HA-NLS(s)

Plasmid

#47867

Purpose

This plasmid encode NmCas9 with two NLS and a HA tag

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

NmCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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Dronpa-Lifeact-7

Plasmid

#54740

Purpose

Localization: Actin, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Article

Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

Insert

Lifeact-Dronpa

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-Mycn-IRES-RFP

Plasmid

#35393

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Mycn (Mycn Mouse)
RFP

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only
pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Mouse Toronto KnockOut (mTKO) CRISPR Library - pLCKO2

Pooled Library

#159393

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Validation (mVAL) CRISPR Library

Pooled Library

#159391

Purpose

Targets 182 immune evasion genes. Designed for loss-of-function screening against genes shown to increase sensistivity or resistance of cancer cells to cytotoxic T lymphocyte-mediated toxicity.

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
pFBOH-MHL_DCAF11:1-546

Plasmid

#210907

Purpose

Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

R207H natural variant (VAR_020121)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_DDB1:1-1140

Plasmid

#210886

Purpose

Insect expression of full-length human DDB1. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140 (DDB1 Human)

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-LIC_GRWD1:1-446

Plasmid

#210876

Purpose

Insect expression of full-length human GRWD1. N-terminal 6X His tag with thrombin protease cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

GRWD1:M1-V446 (GRWD1 Human)

Tags

MGSSHHHHHHSSGLVPRGS

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_DCAF7:1-342

Plasmid

#211118

Purpose

Mammalian expression of full-length human DCAF7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF7:M1-V342 (DCAF7 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_SCAP:1-905

Plasmid

#211228

Purpose

Mammalian expression of full-length human SCAP isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SCAP:M1-D905 (SCAP Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. V798I natural variant (VAR_012203)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_SCAP:1-905

Plasmid

#211302

Purpose

Mammalian expression of full-length human SCAP isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SCAP:M1-D905 (SCAP Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. V798I natural variant (VAR_012203)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_PLCG1:1-1291

Plasmid

#211089

Purpose

Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PLCG1:M1-L1291 (PLCG1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

isoform 2. I813T natural variant (VAR_011908)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CBFA2T3:1-615

Plasmid

#211070

Purpose

Mammalian expression of full-length human CBFA2T3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CBFA2T3:M1-R615 (CBFA2T3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

not annotated isoform

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF11:1-546

Plasmid

#211197

Purpose

Mammalian expression of full-length human DCAF11. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R207H natural variant (VAR_020121)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_RBBP4:1-425

Plasmid

#211153

Purpose

Mammalian expression of full-length human RBBP4. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RBBP4:M1-S425 (RBBP4 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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