We narrowed to 15,035 results for: invs

1. pcDNA3-endo-Nb80

   Plasmid

   #205518

   Purpose

   Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb80
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-EGFP-RhoA-Q63L

   Plasmid

   #12968

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA constitutively active (RHOA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q63L Constitutively active

   Promoter

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. hMEF2A WT

   Plasmid

   #118354

   Purpose

   This plasmid expresses human MEF2A-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-VprBP

   Plasmid

   #133586

   Purpose

   Expresses human VprBP wildtype in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   Vpr (HIV-1) binding protein (DCAF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Wildtype human VprBP (Dcaf1)

   Promoter

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-6xTR-TUBE

   Plasmid

   #110313

   Purpose

   E. coli expression and purification of 6xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   6xTR-TUBE (UBQLN1 Human)
   

   Use

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1 AnxA6

   Plasmid

   #20407

   Depositor

   Carlos Enrich

   Article

   Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

   Insert

   AnxA6 (Anxa6 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-endo-Nb37

   Plasmid

   #205514

   Purpose

   Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb37
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. nLuc-PTHR1

   Plasmid

   #162577

   Purpose

   Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

   Depositor

   Sam Gellman

   Article

   Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

   Insert

   Parathyroid Hormone Receptor 1 (PTH1R Human)
   

   Use

   Tags

   Nanoluc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMYC-GFP

   Plasmid

   #42142

   Depositor

   Olufunmilayo Olopade

   Article

   Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

   Insert

   c-Myc (MYC Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PDE9A_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176945

    Purpose

    Mammalian expression vector encoding PDE9A and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    PDE9A (PDE9A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HDAC7 Flag

    Plasmid

    #13824

    Purpose

    Mammalian expression of human histone deacetylase 7 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC7 (HDAC7 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-PBD-EYFP

    Plasmid

    #13723

    Depositor

    Klaus Hahn

    Article

    Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

    Insert

    PBD-EYFP (PAK1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    p21 binding domain of human Pak1(amino acids 65-1…

    Promoter

    Available Since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-N1 human cofilin S3A

    Plasmid

    #50860

    Purpose

    expresses constituvely active cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3A constitutively active

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3/Myc-DNMT3A

    Plasmid

    #35521

    Purpose

    Mammalian expression of human DNMT3A with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-hERG1a

    Plasmid

    #53052

    Purpose

    Expresses alpha subunit of hERG1a K channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Akt-STOPS

    Plasmid

    #175006

    Purpose

    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. nesprin TSmod 25

    Plasmid

    #125555

    Purpose

    Nesprin-2G force sensor with cerulean-F25-venus TSmodule

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    mini-nesprin-2G (Syne2 Mouse)
    

    Use

    Tags

    cerulean, venus

    Expression

    Mammalian

    Mutation

    TSmod changed from mTFP to cerulean, flageliform …

    Promoter

    CMV

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p4947 pcDNA4c hBrd4 full-length

    Plasmid

    #14441

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (BRD4 Human)
    

    Use

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR26-CMVconst

    Plasmid

    #127373

    Purpose

    Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV enhancer/promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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