We narrowed to 16,204 results for: grna

1. pUD628

   Plasmid

   #103018

   Purpose

   expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crADE2-3.S (ADE2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ131C2.0

   Plasmid

   #99886

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU6

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ132C2.0

   Plasmid

   #99889

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU6

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-PITCh-TUBB4B

   Plasmid

   #207785

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB700 HL1gR4

   Plasmid

   #124450

   Purpose

   Expresses gRNA HL1 gR4 in the pSB700 backbone

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   HL1 gR4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPR-sgUFSP2

   Plasmid

   #86134

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   sgRNA targeting UFSP2 (UFSP2 )
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SGL40C-H1.EFS.RFP657

   Plasmid

   #69148

   Purpose

   Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_B2517

   Plasmid

   #91074

   Purpose

   Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   TaU3

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-GFP-G3BP2(2)

   Plasmid

   #136061

   Purpose

   G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP2 (G3BP2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgLacZs

    Plasmid

    #171185

    Purpose

    Expresses sgRNA

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgLacZ1-hU6-sgLacZ2
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    U6, hSyn

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-U6-crRNA4n96

    Plasmid

    #123640

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 4n96
    

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJZC583

    Plasmid

    #62314

    Purpose

    sgRNA with 2x MS2 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x MS2 binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1154B_gFLE_pB_VTK_ActG

    Plasmid

    #187238

    Purpose

    An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

    Depositor

    Omar Akbari

    Article

    Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

    Insert

    Femaleless
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPN217

    Plasmid

    #91676

    Purpose

    Express sgRNA targeting human TLE1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TLE1 (TLE1 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU1/Sm/3' Box_(GLuc)_INT

    Plasmid

    #68438

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U1

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ132D2.0

    Plasmid

    #99890

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid

    #68432

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJZC593

    Plasmid

    #62319

    Purpose

    sgRNA with MS2-PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + MS2-PP7 RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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