We narrowed to 5,091 results for: codon optimized

1. pHH0103 PIN1L WW domain

   Plasmid

   #104269

   Purpose

   Bacterial expression of WW domain from PIN1L

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   PIN1L WW domain (PIN1P1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTPGI_dCas9_VP64

   Plasmid

   #49013

   Purpose

   encodes yeast-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   pTPGI (galactose+aTc inducible)

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_dCas9_VP64

   Plasmid

   #49015

   Purpose

   encodes human-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (human-codon-optimized)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGC4780

   Plasmid

   #113408

   Purpose

   Translation reporter with inducible translation coupling (high native GFP expression)

   Depositor

   Guillaume Cambray, Adam Arkin

   Article

   Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

   Insert

   158_33121123_1
   

   Use

   Synthetic Biology

   Tags

   fused to codon deptimized sfGFP with Flexible lin…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGC4785

   Plasmid

   #113409

   Purpose

   Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

   Depositor

   Guillaume Cambray, Adam Arkin

   Article

   Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

   Insert

   359_31121113_2
   

   Use

   Synthetic Biology

   Tags

   fused to codon deptimized sfGFP with Flexible lin…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Keasling-2337

   Plasmid

   #100168

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   lacIq-Ptrc-ADS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

   Plasmid

   #163030

   Purpose

   Expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 pA, and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Alb-AAT

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TET-On

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBM019

   Plasmid

   #128179

   Purpose

   Construct for generating stable Cas9-expressing Toxoplasma gondii

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Inserts

   sgRNA#1
   SpCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Keasling-2187

    Plasmid

    #100167

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    MevT and MBIS operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HeFm2SpCas9

    Plasmid

    #92111

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    “Highly enhanced Fidelity” mut2 SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. (517) SB KRAB-SadCas9 U6-gStop

    Plasmid

    #163023

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTO149_RFP_CauNEO

    Plasmid

    #177277

    Purpose

    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    RFP, CauNEO
    

    Use

    Tags

    ENO1-RFP

    Expression

    Bacterial

    Mutation

    All CUG Leucine codons were replaced with the syn…

    Promoter

    AgTEF2

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMYO127TAG-SepT

    Plasmid

    #34625

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin-127TAG
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized + an amber stop codon was introdu…

    Promoter

    Ipp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dm_λHA-FLuc0_CSC

    Plasmid

    #210589

    Purpose

    Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
    

    Use

    Luciferase

    Tags

    lambda HA

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTH849-gst-optec

    Plasmid

    #89590

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-29b(+)_His-CBD-SpEndoH

    Plasmid

    #218196

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

    Depositor

    Florence Pojer

    Insert

    EndoH
    

    Use

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 45-313

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBS-L1PA1CH_blast rescue

    Plasmid

    #69611

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Use

    Tags

    blast rescue cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBHM2641

    Plasmid

    #229525

    Purpose

    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    CRISPR

    Tags

    t2a-mNeonGreen-AID*

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    TEF1

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBHM2647

    Plasmid

    #229989

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBHM2648

    Plasmid

    #229990

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBHM2649

    Plasmid

    #229991

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p426TEF-LptTPS-A

    Plasmid

    #89468

    Purpose

    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production

    Depositor

    Jing-Ke Weng

    Insert

    LptTPS-A
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    Promoter

    pBR322 origin of replication for propagation in E…

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge