Addgene

We narrowed to 19,664 results for: ACE

Showing: 1641 - 1680 of 19664 results

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pRSFDuet1-sgRNA(siteT9)

Plasmid

#154832

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteT9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GolgiCKAR

Plasmid

#14871

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pE_Cre_lambdaCos

Plasmid

#173785

Purpose

The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mutation

S102Y and S279Y- please see depositor comments

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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NucCKAR

Plasmid

#14869

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Use

Tags

CFP, NLS, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pET28CLY5

Plasmid

#21764

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
EZ-L560

Plasmid

#173028

Purpose

Encodes OptoAMP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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DKAR

Plasmid

#14879

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2007 Mar 2. 282(9):6733-42.

Insert

D kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-miR-21 toehold switch

Plasmid

#184756

Purpose

Expresses miR-21 sensor with RFP output

Depositor

Allen Liu

Article

Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

Insert

Red Fluorescence Protein

Use

Tags

miR-21 toehold switch

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9

Plasmid

#64279

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

D10A & H840A relative to S. pyogenes Cas9

Promoter

pTPGI

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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Myr.PKCepsilon.FLAG

Plasmid

#10797

Depositor

Alex Toker

Article

Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

Insert

PKC epsilon (PRKCE Human)

Use

Tags

FLAG and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
EZ-L545

Plasmid

#173024

Purpose

Encodes OptoEXP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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NLS-LgBiT-CaM

Plasmid

#183401

Purpose

Expresses nuclear-localized LgBiT-CaM

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

LgBiT-CaM

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAB203

Plasmid

#101675

Purpose

Opto-T7RNAP(563-F1)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP(563-F1)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-mito-CitroffRH

Plasmid

#158548

Purpose

Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitroffRH

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Magenta

Plasmid

#160446

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Magenta

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-IMX-DogTag

Plasmid

#113765

Purpose

Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

Depositor

Mark Howarth

Article

Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

Insert

IMX-DogTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMK-N. tabacum atpB/accD

Plasmid

#123582

Purpose

MoChlo kit

Depositor

C. Neal Stewart, Scott Lenaghan

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum atpB/accD integration site

Use

Chloroplast transformation vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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EZ-L580

Plasmid

#173023

Purpose

Encodes OptoAMP4 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-J23110-B0034-mRFP1_Yellow

Plasmid

#160443

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Yellow

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pESC/mCitrine

Plasmid

#117996

Purpose

Expression of mCitrine controlled by the Gal1 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

mCitrine

Use

Yeast/bacteria binary vector

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mVenus

Plasmid

#118861

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mVenus

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA12TEC-I

Plasmid

#58308

Purpose

GeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23151

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BLZinCh-1

Plasmid

#85082

Purpose

mammalian vector for the expression of a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging

Depositor

Maarten Merkx

Article

Aper et al ACS Chem Biol. 2016 Oct 21;11(10):2854-2

Insert

BL-ZinCh-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTA-attP

Plasmid

#18939

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 2

Plasmid

#30160

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al Neural Dev. 2008 . 3():15.

Insert

APLP2 shRNA 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pZE-PpadC-yveF-yveG-egfp

Plasmid

#201791

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PpadC-yveF-yveG

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSG84-pLas-TetRssrA

Plasmid

#102455

Purpose

Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pLas

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAB143

Plasmid

#101679

Purpose

LAA-tagged mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

LAA-tagged mCherry under T7 promoter control

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BBa_J72113-BBa_J72103

Plasmid

#40785

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHES840

Plasmid

#89191

Purpose

Expresses YFP under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-YFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM555

Plasmid

#68880

Purpose

NanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ155

Plasmid

#175054

Purpose

psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA12DKR-J

Plasmid

#58306

Purpose

GeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CYPHR

Plasmid

#14864

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

cyan/yellow PH domain reporter (Plcd1 )

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCfB3236

Plasmid

#124708

Purpose

Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS416

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

yEGFP

Use

Tags

Expression

Mutation

Promoter

pTRX2_5xUAS

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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