We narrowed to 59,357 results for: cloning

1. R777-E311 Hs.SHC3

   Plasmid

   #70595

   Purpose

   Gateway ORF clone of codon optimized human SHC3 [NM_016848.5] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   SHC3 (SHC3 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. R777-E270 Hs.RASSF9-nostop

   Plasmid

   #70554

   Purpose

   Gateway ORF clone of human RASSF9 [NM_005447.3] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RASSF9 (RASSF9 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. R777-E280 Hs.RHOB-nostop

   Plasmid

   #70564

   Purpose

   Gateway ORF clone of human RHOB [NM_004040.3] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RHOB (RHOB Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. R777-E282 Hs.RHOC-nostop

   Plasmid

   #70566

   Purpose

   Gateway ORF clone of human RHOC [NM_175744.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RHOC (RHOC Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. R777-E192 Hs.PRKAA1-nostop

   Plasmid

   #70476

   Purpose

   Gateway ORF clone of human PRKAA1 [NM_006251.5] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PRKAA1 (PRKAA1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. R777-E176 Hs.PIK3R3-nostop

   Plasmid

   #70460

   Purpose

   Gateway ORF clone of human PIK3R3 [NM_003629.3] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PIK3R3 (PIK3R3 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R777-E150 Hs.PAK2-nostop

   Plasmid

   #70434

   Purpose

   Gateway ORF clone of human PAK2 [NM_002577.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PAK2 (PAK2 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

   Plasmid

   #224489

   Purpose

   Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Sun1-2xsfGFP-6xMYC-pA
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p5E-MCS-c-Fos-b-globin (JDW 1237)

   Plasmid

   #229829

   Purpose

   A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   c-fos minimal promoter and b-globin intron
   

   Use

   Gateway entry clone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME-EGFP-hs-miR-126 (JDW 1401)

    Plasmid

    #242564

    Purpose

    Gateway middle entry clone containing an EGFP fused to the human microRNA 126.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EGFP with artificial intron encoding miR-126 (MIR126 Human)
    

    Use

    Gateway subcloning

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)

    Plasmid

    #242559

    Purpose

    Gateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
    

    Use

    Gateway subcloning

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME-EGFP-Myc (JDW 1441)

    Plasmid

    #242569

    Purpose

    Gateway middle entry clone containing EGFP fused to oncogenic c-Myc.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    c-Myc (MYC Human)
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

    Plasmid

    #229843

    Purpose

    A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Dll4-F2 (exon3)
    

    Use

    Gateway entry clone

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p5E-Crestin-pro (JDW 1320)

    Plasmid

    #229830

    Purpose

    A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Crestin promoter, c-fos minimal promoter, and b-globin intron
    

    Use

    Gateway entry clone

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid

    #224474

    Purpose

    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

    Plasmid

    #224505

    Purpose

    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC-LexOP-35SCaMV
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

    Plasmid

    #224506

    Purpose

    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-oMDC-LexOP-c-Fos
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

    Plasmid

    #224522

    Purpose

    A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRKH2

    Plasmid

    #71312

    Purpose

    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PiggyBac PlayBack

    Plasmid

    #203312

    Purpose

    To all for PiggyBac gene insertion into Genome

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge