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pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

Plasmid

#61255

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ133-tRNA

Plasmid

#179212

Purpose

Golden Gate entry vector; 3rd gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Promoter

no promoter

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLBH533_MBP-Cas14b2 expression

Plasmid

#112506

Purpose

Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b2

Use

CRISPR

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pT7sgRNA

Plasmid

#111820

Purpose

Expresses sgRNA in T. brucei cells

Depositor

David Horn

Article

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Insert

sgRNA

Use

CRISPR

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9D10A

Plasmid

#128161

Purpose

Ptet-controlled expression of S. pyogenes Cas9D10A variant

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Mutation

D10A

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-AsCas12f

Plasmid

#171612

Purpose

AsCas12f1 heterologous expression plasmid

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-tRNA

Plasmid

#179213

Purpose

Golden Gate entry vector; 4th gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Promoter

no promoter

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSpyB

Plasmid

#220186

Purpose

sgRNA expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only
pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCRL1

Plasmid

#153997

Purpose

sgRNA in vitro transcription plasmid.

Depositor

Shengdar Tsai

Article

Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

Type

Empty backbone

Use

CRISPR; Sgrna under a t7 promoter for in vitro tr…

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZBTB1

Plasmid

#128235

Purpose

Donor vector for 3' FLAG tag of human ZBTB1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFUGW Cas9-BFP

Plasmid

#127396

Purpose

Cas9 expression

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Insert

Cas9

Use

Lentiviral

Tags

BFP

Promoter

SVVF

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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beta2 (px330:2-guideCas9)

Plasmid

#169435

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAWp30

Plasmid

#73857

Purpose

pFUGW-EFSp-Cas9-P2A-Zeo

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

EFSp-Cas9-P2A-Zeo

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ151

Plasmid

#69302

Purpose

Gateway entry vector with pcoCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9D10A (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Tags

NLS-3xHA

Expression

Insect

Promoter

act5C

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBC2103-reci

Plasmid

#117172

Purpose

U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_arCas9 without NLS

Use

Lentiviral

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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