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pKI_ΩBBMVP16&WUS

Plasmid

#220348

Purpose

Expresses BBM-VP16 and WUS in plant cells

Depositor

Tomoko Igawa

Article

Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

Inserts

BABY BOOM (BBM Mustard Weed)
WUSCHEL (WUS Mustard Weed)

Tags

VP16 transcriptional activation domain

Expression

Plant

Promoter

CaMV 35S and RPS5A

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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M51

Plasmid

#242620

Purpose

Strep-MYC-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-MYC-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSLQ9715: pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-Myc_NLS-mCherry-WPRE

Plasmid

#203221

Purpose

Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NFZ-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A

Plasmid

#104447

Purpose

Expression of human VAP-A fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1

Plasmid

#237667

Purpose

For overexpression of mEGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-HsNRF2 (NFE2L2)

Plasmid

#194303

Purpose

Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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ATG-3925 (CBG2)

Plasmid

#167839

Purpose

Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

Depositor

Lance Encell

Article

Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

Insert

Click Beetle Green 2

Tags

6-His

Expression

Bacterial and Mammalian

Mutation

Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

Promoter

CMV, T7

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Article

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSyn

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-osTIR1

Plasmid

#207840

Purpose

Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

osTIR
mCherry
mAID_-VHH-GFP4

Tags

3X HA-Tag and weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40

Plasmid

#110154

Purpose

Expression of human BHLHE40 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-tdTomato-WPRE

Plasmid

#51506

Purpose

Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV5

Insert

tdTomato

Use

AAV

Promoter

phSyn1

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

Plasmid

#234951

Purpose

Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1A

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-FLAG-HA-(N)-CDK12

Plasmid

#231750

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-HA-3C

Expression

Mammalian

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX028

Plasmid

#167151

Purpose

MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnHispS-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX029

Plasmid

#167152

Purpose

MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnH3H-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

Plasmid

#234952

Purpose

Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hTRF2

Plasmid

#103804

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)

Expression

Mammalian

Mutation

hTRF2: deletion of amino acids 1-42 and 476 compa…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLS13-IL-10

Plasmid

#209128

Purpose

Transient mammalian expression of IL-10

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

IL-10 (IL10 Human)

Expression

Mammalian

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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