We narrowed to 1,829 results for: GAD

Showing: 1261 - 1290 of 1829 results

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pmTurquoise2-Mito

Plasmid

#36208

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

MTS (COX8A Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Golgi

Plasmid

#36205

Purpose

In vivo visualization of golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Golgi targeting sequence (B4GALT1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A-L325R

Plasmid

#145373

Purpose

expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

Depositor

Hugues Abriel

Article

Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Expression

Mammalian

Mutation

changed Leucine 325 to Arginine

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-CDC42-G12V-deltaCAAX

Plasmid

#176094

Purpose

Constitutively active, nuclear localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

CDC42 (CDC42 Human)

Expression

Mammalian

Mutation

G12V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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cpVenus-Gγ2

Plasmid

#69626

Purpose

The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

cp173Venus-Ggamma2 (GNG2 Synthetic)

Tags

ciruclar permutated Venus (cp173V)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lck-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129627

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

Lck-cpmTq2-Calcium-lifetime-sensor

Tags

Lck

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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3xnls-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129626

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-cpmTq2-Calcium-lifetime-sensor

Tags

3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RAC1-G12V-deltaCAAX

Plasmid

#176095

Purpose

Constitutively active, nuclear localized Rac1

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RAC1 (RAC1 Human)

Expression

Mammalian

Mutation

G12V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-TGAT(C242S,C253S)

Plasmid

#84336

Purpose

Expresses TGAT tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

TGAT(C242S,C253S) (TRIO Human)

Tags

mCherry

Expression

Mammalian

Mutation

C242S, C253S

Promoter

CMV

Available Since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

Plasmid

#145027

Purpose

Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Use

Lentiviral

Tags

3xnls-Tq-Ca-FLITS and H2B-Maroon

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RHOA-G14V-deltaCAAX

Plasmid

#176097

Purpose

Constitutively active, nucelar localized RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RHOA (RHOA Human)

Expression

Mammalian

Mutation

G14V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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K365-iLid

Plasmid

#188456

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Article

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Tags

6xHis and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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K365-micro

Plasmid

#188457

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Article

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Tags

6xHis-MBP-TEV and SspB micro

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1-2

Plasmid

#52378

Purpose

expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

GAGAL of syndecan-1 replaced with of GADED of SDC…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
STICR_IndexE

Pooled Library

#180483

Purpose

High Complexity Lentiviral Barcode Library

Depositor

Tomasz Nowakowski

Article

Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
STICR_Index1

Pooled Library

#186334

Purpose

High Complexity Lentiviral Barcode Library

Depositor

Tomasz Nowakowski

Article

Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
STICR_Index3

Pooled Library

#186335

Purpose

High Complexity Lentiviral Barcode Library

Depositor

Tomasz Nowakowski

Article

Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
GFP-SMARCA4

Plasmid

#65391

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA4 (SMARCA4 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-AML1/ETO-IRES-GFP

Plasmid

#60832

Purpose

AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

AML1/ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-TAF1

Plasmid

#65395

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TAF1 (TAF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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shERK2b-mlpx-puro

Plasmid

#65230

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK2a-mlpx-puro

Plasmid

#65229

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK1a-mlpx-puro

Plasmid

#65228

Purpose

encodes a shRNA against ERK1

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK2b-mlpx-neo

Plasmid

#65231

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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DOT1L-CC3

Plasmid

#111754

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #3 (residues 613-661)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC1

Plasmid

#111756

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

contains coiled coil #1 (residues 506-555)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC2

Plasmid

#111755

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #2 (residues 560-608)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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