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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 42,249 results for: Ide

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Showing: 8641 - 8660 of 42249 results

  5. pDE43-MCK

    Plasmid
    #49523
    Purpose
    Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49523-plasmid-map-sequence-id-73915

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  7. pETM33_Nsp10

    Plasmid
    #156479
    Purpose
    Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156479-plasmid-map-sequence-id-307731

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  8. HeFSpCas9

    Plasmid
    #92355
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92355-plasmid-map-sequence-id-197617

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  10. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Article
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Insert
    Cerulean linked topaz fluorescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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  14. FRT_htr2c(c)-GFP

    Plasmid
    #79677
    Purpose
    serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP
    Depositor
    Stefan Stamm
    Article
    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do
    Insert
    serotonin receptor 2C (HTR2C Human)
    Tags
    EGFP in Frame
    Expression
    Mammalian
    Mutation
    splice site of exon Vb in consensus
    Promoter
    CMV
    Available Since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. PLG-bio-His

    Plasmid
    #53427
    Purpose
    Expresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PLG (PLG Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53427-plasmid-map-sequence-id-83217

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Showing: 8641 - 8660 of 42249 results