Addgene

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pRPN265NB-enh-MNase

Plasmid

#166035

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

Insert

Glutathione-S-Transferase/enhancer/MNase

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

lac promoter / tac promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SARS-CoV-2 S1 RBD

Plasmid

#167013

Purpose

Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

Expression

Mammalian

Mutation

No stop codon

Promoter

Available since

April 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_PLCG1:1-1291

Plasmid

#211089

Purpose

Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PLCG1:M1-L1291 (PLCG1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

isoform 2. I813T natural variant (VAR_011908)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CBFA2T3:1-615

Plasmid

#211070

Purpose

Mammalian expression of full-length human CBFA2T3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CBFA2T3:M1-R615 (CBFA2T3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

not annotated isoform

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_GEMIN5:1-1508

Plasmid

#211164

Purpose

Mammalian expression of full-length human GEMIN5. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

Gemin5:M1-M1508 (GEMIN5 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW7:1-707

Plasmid

#211099

Purpose

Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW7:1-707

Plasmid

#211104

Purpose

Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW7:M1-K707 (FBXW7 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_RAB7A:1-207

Plasmid

#211095

Purpose

Mammalian expression of full-length human RAB7A. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RAB7A:M1-C207 (RAB7A Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-C_DNAI1:1-699

Plasmid

#211275

Purpose

Mammalian expression of full-length human DNAI1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DNAI1:M1-T699 (DNAI1 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

A8S Y202C P495Q annotated sequence conflicts

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_FBXO3:1-471

Plasmid

#211071

Purpose

Mammalian expression of full-length human FBXO3. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXO3:M1-F471 (FBXO3 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_RPA2:1-270

Plasmid

#211013

Purpose

Mammalian expression of full-length human RPA2. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RPA2:M1-E270 (RPA2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-N_CRBN:1-442

Plasmid

#211066

Purpose

Mammalian expression of full-length human CRBN. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CRBN:M1-L442 (CRBN Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_BTRC:1-605

Plasmid

#211059

Purpose

Mammalian expression of full-length human BTRC. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

BTRC:M1-R605 (BTRC Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CRBN:1-442

Plasmid

#211067

Purpose

Mammalian expression of full-length human CRBN the C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CRBN:M1-L442 (CRBN Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF7:1-342

Plasmid

#211118

Purpose

Mammalian expression of full-length human DCAF7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF7:M1-V342 (DCAF7 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RAB7A:M1-C207 (RAB7A Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_AIRE:1-545

Plasmid

#211094

Purpose

Mammalian expression of full-length human AIRE. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

AIRE:M1-S545 (AIRE Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_BCL11A:1-773

Plasmid

#211014

Purpose

Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

BCL11A:M1-F773 (BCL11A Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_DDB1:1-1140

Plasmid

#211006

Purpose

Mammalian expression of full-length human DDB1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDB1:M1-H1140 (DDB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_DCAF5:1-942

Plasmid

#211200

Purpose

Mammalian expression of full-length human DCAF5. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF5:M1-T942 (DCAF5 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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