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pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAC-BETA

Plasmid

#53272

Purpose

Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

crtE, crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Publication

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pENTR/pTER+ (430-1)

Plasmid

#17453

Purpose

Gateway entry vector for shRNA expression under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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v3em-Cterm-PE2max-∆RNaseH-dualU6

Plasmid

#198735

Purpose

AAV genome encoding C-terminal PE2max and U6 expression cassettes

Depositor

David Liu

Publication

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

NpuC-CtermPE2max∆RNaseH

Use

AAV

Promoter

Cbh

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pQLinkH

Plasmid

#13667

Depositor

Konrad Buessow

Publication

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

His, TEV, and attB1

Expression

Bacterial

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pDEST51-LRRK2-G2019S

Plasmid

#29401

Depositor

Mark Cookson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Gly2019Ser

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PB-TA-ERP2

Plasmid

#80477

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Publication

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Publication

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM28 (TRIM28 Human)

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Publication

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSyn

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Publication

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBa.TfR.GFP

Plasmid

#45060

Depositor

Gary Banker, Marvin Bentley

Publication

Burack et al Neuron. 2000 May;26(2):465-72.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TfR (TFRC Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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HA-EglN1-pcDNA3

Plasmid

#18963

Depositor

William Kaelin

Publication

Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Egg laying Nine - 1 (EGLN1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Publication

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tau AP (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP-CFAST11

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Publication

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pSLTS

Plasmid

#59386

Purpose

Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

Depositor

Shelley Copley

Publication

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Type

Empty backbone

Use

Genome engineering

Expression

Bacterial

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST-CMV-3xFLAG-gateway-EGFP

Plasmid

#122845

Purpose

N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRK5 TGF beta type I receptor (T202D) Flag

Plasmid

#14833

Depositor

Rik Derynck

Publication

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

flag

Expression

Mammalian

Mutation

Threonine 202 mutated to aspartate. Constitutivel…

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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