We narrowed to 14,915 results for: mal.3
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Plasmid#82705PurposeAAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertU6/TO gRNA Expression Cassette Containing Tet2 gRNA
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
155_KRAS in pmiRGlo
Plasmid#78132Purposeluciferase reporter for miRNA activityDepositorInsertKRAS (KRAS Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
YFP(159-238)-gamma-2 in pcDNAI/Amp
Plasmid#54472PurposeA carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertYFP(159-238)-gamma -2 (GNG2 Human, Aequorea victoria)
TagsYFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationGgamma-2 was amplified by PCR, which added a BamH…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-1 in pcDNAI/Amp
Plasmid#54468PurposeAn amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
TagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
ROZA-XL
Plasmid#64194PurposeFluorescent reporter for ZAP-70 tyrosine kinase activityDepositorInsertROZA-XL
ExpressionMammalianMutationROZA-XLPromoterCMVAvailable SinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-delta6xNHL-LIN-41
Plasmid#52722Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 593–868Available SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)
Plasmid#38262DepositorInsertβ-arrestin2 (Arrb2 Rat)
TagsFlag and PIP5K Iα core kinase domain (residues 18…ExpressionMammalianMutationArg 233, Arg237, and Lys251 converted to Glutamin…PromoterCMVAvailable SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(FF)
Plasmid#27135DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationTyrosines in ITAMS 1-3 mutated to phenylalanines …Available SinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
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-111 hTF m2
Plasmid#15449DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseExpressionMammalianMutationAll 3 Sp1 sites mutatedAvailable SinceAug. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOPTN1-424-EGFP
Plasmid#68840Purposedeleted OPTN was inserted into pEGFP-N1 backboneDepositorAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPTN1-55-EGFP
Plasmid#68838Purposedeleted OPTN was inserted into pEGFP-N1 backboneDepositorAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-1P-Kv-WPRE
Plasmid#202612PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSynDepositorInsertJEDI-1P-Kv
UseAAVExpressionMammalianPromoterhSynAvailable SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA
Plasmid#110624PurposeA single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNADepositorInsertsSt1Cas9 LMD-9
sgRNA for St1Cas9
UseAAV, CRISPR, and Mouse TargetingTagsSV40 NLSExpressionMammalianPromoterLP1B and hU6Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N3::hTET3 HxD
Plasmid#136365PurposeExpresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cellsDepositorInsertTET3 (TET3 Human)
Tags2xStrepTagII, FLAG, and HAExpressionMammalianMutationHxD (H1077Y, D1079A)Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-KDM6B-U
Plasmid#255434PurposeMammalian expression of HA-tagged human JMJD3 with its own 3'UTRDepositorAvailable SinceMay 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_nLightR2-control
Plasmid#221675PurposeExpresses nLightR2-control in neuronsDepositorInsertnLightR2-control
UseAAVExpressionMammalianPromoterhuman Synapsin-1Available SinceFeb. 26, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcg 2.0-BFP-circ-arRNA backbone
Plasmid#213965Purposepcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences oDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128587PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)
Plasmid#71214PurposedCas9 fused to BFP and the human DNMT3B catalytically inactive domainDepositorInsertdSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
TagsdSpCas9-BFP-DNMT3B(E697A)ExpressionMammalianAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only