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We narrowed to 15,041 results for: pET

Showing: 2201 - 2250 of 15041 results

  1. pET21-pelB-LaG-9

    Plasmid
    #172742
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-9
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172742-plasmid-map-sequence-id-340182

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  5. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Article
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6×His Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Promoter
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  10. pET21-pelB-LaG-8

    Plasmid
    #172761
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-8
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172761-plasmid-map-sequence-id-340116

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  11. pET21-pelB-LaG-5

    Plasmid
    #172760
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-5
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172760-plasmid-map-sequence-id-340022

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  17. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid
    #127637
    Purpose
    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.
    Depositor
    Friedrich Simmel
    Article
    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.
    Insert
    YPet
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PP7 coat protein (PCP)
    Expression
    Bacterial
    Mutation
    Promoter
    pJ21306
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127637-plasmid-map-sequence-id-251705

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  25. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid
    #122849
    Purpose
    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT) (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122849-plasmid-map-sequence-id-238542

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  26. pETM-30-GST-Dmp68_B

    Plasmid
    #145962
    Purpose
    Bacterial Expression of Dmp68
    Depositor
    Elisa Izaurralde
    Insert
    Dmp68 (Rm62 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145962-plasmid-map-sequence-id-382819

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  27. pETM6-3F2-mCherry

    Plasmid
    #73418
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73418-plasmid-map-sequence-id-135318

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  29. pBP-pET-RBS

    Plasmid
    #72981
    Purpose
    PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    pET RBS derived from pET vectors
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72981-plasmid-map-sequence-id-447503

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  37. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid
    #133970
    Purpose
    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.
    Depositor
    Pia Lindberg
    Article
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    mVenus
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available Since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133970-plasmid-map-sequence-id-267750

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  41. pET32a-His-FADD

    Plasmid
    #78803
    Purpose
    To purify His-tagged recombinant FADD protein
    Depositor
    Jaewhan Song
    Insert
    GIG3 (FADD Human)
    Use
    Tags
    6x His, S-Tag, Thrombin cleavage site, and TrxA (…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  42. pET-PesaR-Plux

    Plasmid
    #47659
    Purpose
    PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)
    Depositor
    Cynthia Collins
    Article
    Shong et al ACS Synth Biol. 2013 Jul 23.
    Insert
    PesaR-P
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Additional esa box downstream of the original esa…
    Promoter
    PesaR-P
    Available Since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  46. ymScarletI-YPET pDRF1-GW

    Plasmid
    #168063
    Purpose
    FRET fusion between ymScarletI and YPET for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymScarletI-YPET
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168063-plasmid-map-sequence-id-332368

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  48. pET21-pelB-LaG-2

    Plasmid
    #172739
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-2
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172739-plasmid-map-sequence-id-340192

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  50. pET11a-p52(1-398)

    Plasmid
    #44733
    Depositor
    Gourisankar Ghosh
    Insert
    p52 (NFKB2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    AA 1-398
    Promoter
    T7
    Available Since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 2201 - 2250 of 15041 results