We narrowed to 3,332 results for: UBC

1. pAW-BiP-NbVHH05-mCherry-KDEL

   Plasmid

   #171574

   Purpose

   Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
   

   Use

   Tags

   BiP signal peptide and mCherry-KDEL

   Expression

   Insect

   Mutation

   Promoter

   fly actin5C promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-HGP-v3-NbVHH05-hIgG

   Plasmid

   #171565

   Purpose

   Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
   

   Use

   Tags

   BiP signal peptide and hIgG1-hinge-N297A-TEV site…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-HA-Ubiquitin-K48

   Plasmid

   #17605

   Purpose

   Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K48 only, other lysines mutated to arginines. Enh…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-Ubiquitin-K63

   Plasmid

   #17606

   Purpose

   Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K63 only, other lysines mutated to arginines. Enh…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E ubb polyA

   Plasmid

   #195977

   Purpose

   Gateway p3E vector

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubb:polyA (ubb Zebrafish)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K33

   Plasmid

   #17607

   Purpose

   Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K33 only, other lysines mutated to arginines; P19…

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-hSNCA-GFP

   Plasmid

   #215489

   Purpose

   Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fuw-dCas9-dead Tet1CD-P2A-BFP

   Plasmid

   #108246

   Purpose

   Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-dead Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

   Promoter

   Ubc

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA-Ubiquitin-K6

   Plasmid

   #121151

   Purpose

   Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All Ks apart from K6 mutated to Rs

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-hSNCA-NE

    Plasmid

    #215486

    Purpose

    Lentiviral expression of human alpha-synuclein in mammalian cells

    Depositor

    Ophir Shalem

    Article

    Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Lentiviral

    Tags

    NE tag

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-HA-Ubiquitin-K11R

    Plasmid

    #121154

    Purpose

    Mammalian expression of HA tagged ubiquitin K11R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K11 mutated to an R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-HA-Ubiquitin-K11

    Plasmid

    #121152

    Purpose

    Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All Ks apart from K11 mutated to Rs

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-Ubiquitin-K27R

    Plasmid

    #121155

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K27 mutated to an R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-Ubiquitin-K6R

    Plasmid

    #121153

    Purpose

    Mammalian expression of HA tagged ubiquitin K6R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K6 mutated to an R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2CG2-ubi:QFGal4-SV40pA

    Plasmid

    #155119

    Purpose

    Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    ubi:QFGal4 (ubb Zebrafish, Neurospora crassa, Budding Yeast)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    ubiquitin B

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEN25-Ub Hs

    Plasmid

    #83232

    Purpose

    pHis-par2-Ub Hs

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Ubiqutin (UBC Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-Ubiquitin-K27R-K29R

    Plasmid

    #121156

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R and K29R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K27 and K29 mutated to Rs

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CNTF-HA

    Plasmid

    #195517

    Purpose

    Expresses HA-tagged CNTF in mammalian cells

    Depositor

    Kevin Park

    Article

    Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

    Insert

    Ciliary neurotrophic factor (CNTF Human)
    

    Use

    AAV

    Tags

    HA-tag and NGF signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    UbC promoter with beta-globin intron

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-module1

    Plasmid

    #222838

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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