We narrowed to 60,006 results for: %s

1. pcDNA3-Lyn-dInPAkt(R23/25A)

   Plasmid

   #181835

   Purpose

   Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-dInPAkt
   

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Mutation

   Arginines 23 and 25 in PH domain mutated to alani…

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Calnexin-HT-mGold

   Plasmid

   #183987

   Purpose

   Expresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   Calnexin (CANX Human)
   

   Tags

   HaloTag, mGold

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRvSAHS1-mEGFP-NLS

   Plasmid

   #205005

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRvCAHS3-mEGFP-NLS

   Plasmid

   #205004

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-EGFP-Rab5

   Plasmid

   #174454

   Purpose

   Express EGFP with early endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with early endosome-targeting sequence
   

   Tags

   small GTPase (Rab5)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCALNL-NaChBac

   Plasmid

   #125580

   Purpose

   Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   NaChBac
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. #2.4 pre-G-SynapShot-Myc

   Plasmid

   #214722

   Purpose

   Expresses green #2.4 pre-SynapShot and Myc tag

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #2.4 pre-G-SynapShot
   

   Use

   AAV

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Wnt4-V5

   Plasmid

   #35929

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt4 (WNT4 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Silent Mutation C (492) --> T

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSC1-GiP

   Plasmid

   #58254

   Purpose

   This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Human EF1-alpha

   Available Since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI HA NEDD4L DD

    Plasmid

    #27001

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4L (NEDD4L Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C962A

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid

    #84247

    Purpose

    Expresses direct fusion VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, VPR, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR TDP43 WT YFP

    Plasmid

    #27470

    Depositor

    Aaron Gitler

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSN23

    Plasmid

    #100108

    Purpose

    expresses a chimeric SOX2-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to a 'medium' VP16 domain (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AWP-029

    Plasmid

    #213966

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Segatella bryantii, Synthetic)
    

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-SpiCee-NLS

    Plasmid

    #140900

    Purpose

    mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NLS
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+N-eGFP_SAMD9L

    Plasmid

    #206173

    Purpose

    mammalian expression of WT SAMD9L

    Depositor

    Christopher Goodnow

    Article

    Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

    Insert

    SAMD9L (SAMD9L Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PL6-CS-WR

    Plasmid

    #122512

    Purpose

    construction for sgRNA transcription with Plasmodium falciparum U6 promotor

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    PfU6

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFX-iRFP-Rab7

    Plasmid

    #174459

    Purpose

    Express iRFP with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with late endosome-targeting sequence
    

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-mPERK-FLAG-IP

    Plasmid

    #101793

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse PERK

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Perk (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIP CMV VHHG4

    Plasmid

    #182236

    Purpose

    Expresses GFP nanobody for stable expression in mammalian cells

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    His and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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