We narrowed to 110,724 results for: %s

1. SpCas9-NRCH-ABE8.17-m+V106W

   Plasmid

   #198551

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE3G-GSX2

   Plasmid

   #96964

   Purpose

   Dox-inducible expression of Gsx2 in mammalian cells

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GSX2 (Gsx2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-ChRmine-eYFP-WPRE

   Plasmid

   #130988

   Purpose

   AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PRPF19

   Plasmid

   #155847

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PRPF19 (PRPF19 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1014 pcDNA3 T7 Akt1 K179M T308A S473A

   Plasmid

   #9031

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-myc-his-BACH1 K52R

   Plasmid

   #17643

   Depositor

   Ronny Drapkin

   Article

   Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

   Insert

   BACH1 (BRIP1 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   The lysine residue at position 52 was changed to …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

   Plasmid

   #172104

   Purpose

   Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM643

   Plasmid

   #112533

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   luxCDABE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17P

   Promoter

   J23107, PL(HrtO), and ProD

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3 basic Mut E2

   Plasmid

   #48748

   Purpose

   Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutated E-box2 sequence from CACGTT to GCTAGT

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. PRPF3

    Plasmid

    #155848

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF3 (PRPF3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTET2-RlucCP-3MS2

    Plasmid

    #198354

    Purpose

    Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTET2

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJR019

    Plasmid

    #198834

    Purpose

    Inactivation of G protein subunits through Pertussis toxin (PTX)

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::PTX-SL2-GFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. 902 pLNCX myr HA Akt1 K179M

    Plasmid

    #9006

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    K179M. kinase dead.

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-CHAMP1

    Plasmid

    #159892

    Purpose

    Expresses CHAMP1 (CAMP) in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

    Insert

    CHAMP1 (CHAMP1 Human)
    

    Use

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. NSUN2

    Plasmid

    #155793

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    NSUN2 (NSUN2 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST1374-scFv-APOBEC-UGI-GB1

    Plasmid

    #113029

    Purpose

    Expresses scFv-rAPOBEC1-UGI in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    scFv-rAPOBEC1-UGI-GB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-puro-mycRSK2wt

    Plasmid

    #15827

    Depositor

    Jing Chen

    Article

    Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

    Insert

    Ribosomal S6 kinase 2 (Rps6ka3 Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. SF3A2

    Plasmid

    #156001

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3A2 (SF3A2 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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