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We narrowed to 3,228 results for: Streptococcus

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Showing: 901 - 920 of 3228 results

  8. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  14. pDIV490

    Plasmid
    #177698
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177698-plasmid-map-sequence-id-352588

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  15. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  16. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

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  20. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid
    #60600
    Purpose
    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Inserts
    CRISPR Cas9 D10A
    puromycin resistance gene
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed Asp 10 to Ala, Codon usage optimized for …
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    60600-plasmid-map-sequence-id-97110

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Showing: 901 - 920 of 3228 results