We narrowed to 14,357 results for: cas9

Showing: 901 - 920 of 14357 results

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pBWB424

Plasmid

#140638

Purpose

RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

Depositor

Keith Tyo

Publication

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Expression

Mammalian

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SiC-V1-Scr

Plasmid

#133042

Purpose

SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

Depositor

Sriram Neelamegham

Publication

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUS2-SH231

Plasmid

#115150

Purpose

Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

Depositor

Raymond Monnat, Michael Phelps

Publication

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dual SH231 gRNAs

Use

CRISPR

Tags

3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

Expression

Mammalian

Promoter

U6, CBh

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 5

Plasmid

#51764

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 5

Use

CRISPR and Lentiviral

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28b-NmCas9-Flag H588A

Plasmid

#109032

Purpose

H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Publication

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag D16A+H588A

Plasmid

#109033

Purpose

D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Publication

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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EC70197

Plasmid

#209458

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70204

Plasmid

#209465

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70188

Plasmid

#209449

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

Plasmid

#101205

Purpose

Bacterial expression plasmid for SpCas9 REC3 domain

Depositor

Jennifer Doudna, Keith Joung

Publication

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K506–Q712

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K506–Q712

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

Plasmid

#97313

Purpose

Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

Lentiviral and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

Plasmid

#49772

Purpose

Level 2 construct

Depositor

Sophien Kamoun

Publication

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

sgRNA_PDS2-Cas9-sgRNA_PDS1

Use

CRISPR

Expression

Plant

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Mouse sgRNA library Brie in lentiGuide-Puro

Pooled library

#73633

Purpose

Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

68 Articles
DS-ST1cas

Plasmid

#48647

Purpose

Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
ST1 tracrRNA

Use

CRISPR

Promoter

proC

Available since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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Sniper-Cas9

Plasmid

#138559

Purpose

Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

Sniper-Cas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

F539S, M763I, K890N

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CASI-Cas9C-P2A-turboGFP

Plasmid

#80942

Purpose

Expresses Cas9C in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Publication

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 puro

Plasmid

#98290

Purpose

Variant of lentiCRISPRv2 that confers puromycin resistance

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

267 Articles

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Publication

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pSQT817

Plasmid

#53373

Purpose

wildtype Cas9 expression plasmid

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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