We narrowed to 8,996 results for: App;

1. pJK528

   Plasmid

   #71625

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes methionine-105 to serine, isoleucine-109 …

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK439

   Plasmid

   #72478

   Purpose

   Produces Thermoplasma acidophilum citrate synthase pseudo-dimer

   Depositor

   T. J. Kappock

   Insert

   citrate synthase (artificial dimer)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   inserted NSTCLVPRGSPV sequence between C- and N-t…

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK404

   Plasmid

   #72417

   Purpose

   Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   acyl-CoA thioesterase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   additional N-terminal 15 residues MSETYGTGQKEQHC,…

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK268

   Plasmid

   #72436

   Purpose

   Produces Acetobacter aceti 1023 del7-GloA

   Depositor

   T. J. Kappock

   Insert

   lactoylglutathione lyase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Missing residues 1-7

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK627

   Plasmid

   #72462

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to aspartate, tyrosine…

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK628

   Plasmid

   #72463

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to alanine, tyrosine-1…

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK573

   Plasmid

   #72456

   Purpose

   Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   synthetic gene with ~57 silent substitutions

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK632

   Plasmid

   #72465

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to valine, tyrosine-15…

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK629

   Plasmid

   #72464

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to threonine, tyrosine…

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK541

    Plasmid

    #72447

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK569

    Plasmid

    #72455

    Purpose

    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    synthetic gene with ~51 silent substitutions

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK587

    Plasmid

    #71696

    Purpose

    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase 1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-138 to serine

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK525

    Plasmid

    #71601

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tryptopha…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK526

    Plasmid

    #71602

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-97 to tyrosine, tryptophan-165…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK601

    Plasmid

    #71606

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe mutant (AaPurE1-Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tyrosine-154 to phenylalanine, additional…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK698

    Plasmid

    #71616

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tryptopha…

    Promoter

    T5

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK507

    Plasmid

    #71597

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, tryptopha…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK509

    Plasmid

    #71599

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn mutant (AaPurE1fww-H59N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK021

    Plasmid

    #75060

    Purpose

    Produces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E. coli optimized codons in first 25 residues

    Promoter

    lac

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK642

    Plasmid

    #72391

    Purpose

    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)

    Depositor

    T. J. Kappock

    Insert

    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutated tyrosine-397 to phenylalanine to block ad…

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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