We narrowed to 8,785 results for: sgrna

1. pLentiCrisprV2 sgRNA hCRY1 c-term

   Plasmid

   #179453

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human CRY1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMD19-PBBa_J23117-sgRNA-Spr

   Plasmid

   #190793

   Purpose

   Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA (dummy)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23117

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

   Plasmid

   #122333

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBx-Spas-sgRNA-Kan

   Plasmid

   #105233

   Purpose

   Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNTI661 pRPR1(TetO)-sgRNA

   Plasmid

   #139475

   Purpose

   Vector for tet-regulated sgRNA expression in yeast

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Insert

   single guide RNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pRPR1(TetO)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

   Plasmid

   #122334

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUb-Cas9-mCherry_cU6:6-sgRNA

   Plasmid

   #190598

   Purpose

   The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   mCherry - separated by T2A

   Expression

   Mutation

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX335-NQL002-WAPL-sgRNA1

   Plasmid

   #175550

   Purpose

   For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nickase and sgRNA against mouse WAPL STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX335-NQL003-WAPL-sgRNA2

    Plasmid

    #175551

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nickase and sgRNA against mouse WAPL STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro U6 sgRNA CAG

    Plasmid

    #50927

    Purpose

    U6 driven sgRNA negative control

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    negative control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

    Plasmid

    #218666

    Purpose

    sgRNA targeting human EIF2AK3/PERK

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

    Plasmid

    #218528

    Purpose

    sgRNA targeting human EIF2AK4/GCN2

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-sp-sgRNA-mDNMT1_nick-lenti

    Plasmid

    #135959

    Purpose

    S. pyogenes nicking sgRNA for mouse DNMT1

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    mDNMT1_nick
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUF-Cas9-pre-sgRNA

    Plasmid

    #137845

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6-pegRNA-H1-nick sgRNA-mCherry

    Plasmid

    #176901

    Purpose

    Expresses prime editing guide RNA and guide RNA in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Inserts

    mCherry
    gRNA scaffold
    gRNA scaffold
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, H1, and U6

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid

    #154196

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC1-A, sgRNA-BC1-C
    

    Use

    Lentiviral; Catch barcode activation

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and mU6

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-sgRNA-Cre-GpNLuc

    Plasmid

    #208382

    Purpose

    Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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