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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,008 results for: T7

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Showing: 9561 - 9580 of 11008 results

  2. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid
    #101229
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101229-plasmid-map-sequence-id-197216

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  15. pET28a-6xHis-SSF1

    Plasmid
    #255298
    Purpose
    SSF1 (1-473) with an N-terminal 6X poly-histidine tag and a TEV recognition site sub-cloned into pET28 plasmids with Kanamycin bacterial resistance, were used for protein expression
    Depositor
    Richard Kriwacki
    Article
    Dogra et al bioRxiv 2025.03.01.640913
    Insert
    SSF1 (PPAN Human)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255298-plasmid-map-sequence-id-509456

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  16. pET28a-6xHis-SURF6-C19S

    Plasmid
    #255297
    Purpose
    Single cysteine mutant (C19S) of SURF6 (1-361) with an N-terminal 6X poly-histidine tag and a TEV recognition site sub-cloned into pET28 plasmids with Kanamycin bacterial resistance.
    Depositor
    Richard Kriwacki
    Article
    Dogra et al bioRxiv 2025.03.01.640913
    Insert
    SURF6-C19S (SURF6 Human)
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Mutated cysteine 19 to serine
    Promoter
    T7
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255297-plasmid-map-sequence-id-509450

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  17. pET28a-6xHis-SURF6

    Plasmid
    #255296
    Purpose
    SURF6 (1-361) with an N-terminal 6X poly-histidine tag and a TEV recognition site sub-cloned into pET28 plasmids with Kanamycin bacterial resistance, were used for protein expression.
    Depositor
    Richard Kriwacki
    Article
    Dogra et al bioRxiv 2025.03.01.640913
    Insert
    SURF6 (SURF6 Human)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    255296-plasmid-map-sequence-id-509016

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Showing: 9561 - 9580 of 11008 results