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RNT1-GFP

Plasmid

#17708

Depositor

Hal Dietz

Article

Mendell et al Science. 2002 Oct 11. 298(5592):419-22.

Insert

rent1 (UPF1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-Dronpa

Plasmid

#182332

Purpose

AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

AKAP79-Dronpa (AKAP5 Human)

Tags

Full-length AKAP79

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-C-TEV-Twin-Strep

Plasmid

#202527

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.ELREA_746del

Plasmid

#82911

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

Deletion of residues 746-750 (ELREA)

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GFP2-NbALFA

Plasmid

#201486

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

GFP2-NbALFA

Tags

GFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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qs5

Plasmid

#24498

Depositor

Bruce Conklin

Article

Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

Insert

Gq alpha (Gnaq Mouse)

Tags

HA (see comments)

Expression

Mammalian

Mutation

Replacement of the five carboxyl-terminal amino a…

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
G10 Cas9 MDC123

Plasmid

#59187

Purpose

G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

G10

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ucp1-empty-miR122

Plasmid

#231695

Purpose

Empty control vector for pAAV-Ucp1-Cre-miR122

Depositor

Joerg Heeren

Article

Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

Type

Empty backbone

Use

AAV

Promoter

rat Ucp1 enhancer-promoter

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pγsat

Plasmid

#39238

Depositor

Niall Dillon

Article

Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

Insert

8 copies of the 234bp mouse γ-satellite repeat

Use

Cloning vector

Available Since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
pVITRO1-102.1F10-IgA1/λ

Plasmid

#50370

Purpose

Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TGFbeta receptor I K232R

Plasmid

#11763

Depositor

Jeff Wrana

Article

Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

Insert

TGFbeta receptor I (TGFBR1 Human)

Tags

6xHIS

Expression

Mammalian

Mutation

K232R: kinase dead

Promoter

CMV

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+) ZKSCAN1 Sense

Plasmid

#60631

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight

Plasmid

#45150

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight

Tags

DsRed and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMpGWB301

Plasmid

#68629

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

Plasmid

#169550

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette

Expression

Mammalian

Promoter

PPGK

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB101

Plasmid

#68555

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HshnRNPQ_AA

Plasmid

#148251

Purpose

Mammalian Expression of HshnRNPQ

Depositor

Elisa Izaurralde

Insert

HshnRNPQ (SYNCRIP Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaD (pESC106)

Plasmid

#50388

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

PPAT

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CMVs.TurboRFP.WPRE.RBG (AAV8)

Viral Prep

#105548-AAV8

Purpose

Ready-to-use AAV8 particles produced from pENN.AAV.CMVs.TurboRFP.WPRE.RBG (#105548). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.TurboRFP.WPRE.RBG plasmid DNA. CMV-driven TurboRFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

James M. Wilson

Promoter

CMV

Tags

TurboRFP

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only