We narrowed to 4,283 results for: 261
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Plasmid#63829PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-5
Plasmid#63830PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-6
Plasmid#63831PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-7
Plasmid#63832PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-8
Plasmid#63833PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-9
Plasmid#63834PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-11
Plasmid#63836PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL671
Plasmid#62825PurposeLim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsglgC leaderExpressionBacterialMutationPromoterpConAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1017
Plasmid#62828PurposeLim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsm8::glgC leaderExpressionBacterialMutationPromoterpConNoHindAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-CENPBi2
Plasmid#59619PurposeExpression of shRNA against human CENPBDepositorInsertCENPB (CENPB Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX21
Plasmid#46849PurposeGene targeting to generate Trp53 Platform (PLF) mouseDepositorInsert129/Ola mouse Trp53 gene, Puro and attP sites
UseMouse TargetingTagsExpressionMutationattP flanked PGK-NeoPromoterAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAGI1
Plasmid#38882PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAGI1 (MAGI1 Human)
UseTagsHis TevExpressionBacterialMutationC890S; codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAGI1
Plasmid#38883PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAGI1 (MAGI1 Human)
UseTagsHis TevExpressionBacterialMutationC1189S; codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAGI1
Plasmid#38901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAGI1 (MAGI1 Human)
UseTagsHis TevExpressionBacterialMutationC677S, C709S; codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas12a-recX
Plasmid#158714PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recXDepositorInsertrecX
UseCRISPRTagsExpressionBacterialMutationPromoterPmycAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRTagsExpressionBacterialMutationPromoterTetOAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
TL026_AAV_hSyn:SEP-GluA1
Plasmid#192519PurposeExpression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusionDepositorInsertSEP-GluA1
UseAAV and Cre/LoxTagssuper ecliptic phluorinExpressionMutationPromoterhSynAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pREDCas9
Plasmid#71541PurposeConstitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
lambda red genes
plasmid curing system
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-IntCCCre-T2A-CopGFP
Plasmid#160501PurposeExpresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsCopGFPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLX_307 GFP
Plasmid#184492PurposeLentivirus expression for GFPDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-C1-ER alpha
Plasmid#28230PurposeMammalian expression of Estrogen Receptor alpha fused to EGFPDepositorInsertEstrogen Receptor alpha (ESR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXP1
Plasmid#153145PurposeExpression of Flag tagged human FOXP1 in mammalian cellsDepositorInserthuman FOXP1 (FOXP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXK2
Plasmid#153133PurposeExpression of Flag tagged human FOXK2 in mammalian cellsDepositorInserthuman FOXK2 (FOXK2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
perbB2-EGFP
Plasmid#39321DepositorInserterbB2 (ERBB2 Human)
UseTagsegfpExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138131PurposeExpresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertmCherry-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFC332
Plasmid#87845PurposeAMA1 plasmid with Aspergillus optimized Cas9 and hph selection markerDepositorInsertsCas9
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC333
Plasmid#87844PurposeAMA1 plasmid with Aspergillus optimized Cas9 and ble selection markerDepositorInsertsCas9
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXO1A
Plasmid#153141PurposeExpression of Flag tagged human FOXO1A in mammalian cellsDepositorInserthuman FOXO1 (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A
Plasmid#41999DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXP3
Plasmid#153147PurposeExpression of Flag tagged human FOXP3 in mammalian cellsDepositorInserthuman FOXP3 (FOXP3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only