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pRN227

Plasmid

#127222

Purpose

BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Cas9, WUS2, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAd nGFP

Plasmid

#19413

Depositor

Douglas Melton

Publication

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

nuclear GFP

Use

Adenoviral and Gateway Cloning

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGBMCS-SortA

Plasmid

#21931

Depositor

Fuyuhiko Inagaki

Publication

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase (srtA Staphylococcus aureus (strain N315))

Tags

GB1 and His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
iRFP-P4M-SidM

Plasmid

#51470

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Publication

Hammond et al J Cell Biol. 2014 Apr 7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

P4M-SidM(546-647)

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKmyc-Exp5

Plasmid

#12552

Depositor

Ian Macara

Publication

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Exportin-5 (XPO5 Human)

Tags

Myc

Expression

Mammalian

Mutation

None

Available since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LeGO-G2

Plasmid

#25917

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, eGFP

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Publication

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

10xHis and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Publication

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B

Plasmid

#104448

Purpose

Expression of human VAP-B fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Publication

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28a-SrtAdelta59

Plasmid

#51138

Purpose

expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

Depositor

Hidde Ploegh

Publication

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.aureus SrtA delta 59

Tags

6x His

Expression

Bacterial

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
Pet28a_Human_H4

Plasmid

#42633

Depositor

Joe Landry

Insert

Human H4 (H4C1 Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pU6b:sgRNA#3

Plasmid

#64247

Purpose

expresses sgRNA under U6b promoter

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET28b-BE1

Plasmid

#73018

Purpose

Expresses BE1 with N-terminal His6 tag in E. Coli

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Publication

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAdTrack-CMV FLAG Rev-Erb alpha

Plasmid

#22745

Depositor

Bruce Spiegelman

Publication

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

Rev Erb alpha (NR1D1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Publication

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Publication

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Publication

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

128 Articles

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pAd PdxI-I-nGFP

Plasmid

#19411

Depositor

Douglas Melton

Publication

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

pancreatic and duodenal homeobox 1 (Pdx1 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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