We narrowed to 35,319 results for: TES

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pCDNA3_PKA-CαN-mPAGFP

Plasmid

#114114

Purpose

Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-TAK1 5'-UTR

Plasmid

#115356

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNA

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

TAK1 5'-UTR (MAP3K7 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-KD

Plasmid

#114066

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-KD (TTK Human)

Tags

GST

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-WT

Plasmid

#114065

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-WT (TTK Human)

Tags

GST

Expression

Insect

Mutation

none

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-M4

Plasmid

#114036

Purpose

Expression of HEC1 M4 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M4 (NDC80 Human)

Expression

Mammalian

Mutation

M4 (K146E, R153E, K156E)

Promoter

CMV TetO

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-M2

Plasmid

#114035

Purpose

Expression of HEC1 M2 mutant tagged with GFP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

GFP tagged - HEC1 -M2 (NDC80 Human)

Expression

Mammalian

Mutation

M2 (K81E, N87E, K89E)

Promoter

CMV TetO

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)

Plasmid

#112802

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 14_22 and 447_454 deleted

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 PUM FL

Plasmid

#112997

Purpose

For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

pumilio (pum Fly)

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-C-SH2

Plasmid

#111419

Purpose

express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

Insert

murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-C-SH2+IA

Plasmid

#111418

Purpose

express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

Insert

murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-48-GFP

Plasmid

#108507

Purpose

expression of vsv-INCENP 1-48-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-58-GFP

Plasmid

#108508

Purpose

expression of vsv-INCENP 1-58-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-63-GFP

Plasmid

#108509

Purpose

expression of vsv-INCENP 1-63-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-vsv-Mis12-dCEN-Incenp-EGFP

Plasmid

#108478

Purpose

expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 N1(1-259)

Plasmid

#89830

Purpose

Express GATA3 N1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

N1 (AA 1-259)

Promoter

CMV

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(295-443)

Plasmid

#90316

Purpose

Express GATA3 C2 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

GATA3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C2 (AA 295-443)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 N2(1-294)

Plasmid

#89831

Purpose

Express GATA3 N2 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

N2 (AA 1-294)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP7.v2 puro

Plasmid

#87795

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

Plasmid

#87776

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4 (MAP2K4 Human)

Tags

3xFLAG and NLS

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR (EGFP) shPnky-2

Plasmid

#79142

Purpose

Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

Depositor

Daniel Lim

Article

Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

Insert

shPnky-2 (Gm30731 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for the shRNA and CMV for EGFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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