We narrowed to 23,671 results for: CRISPR

1. pGEM-T Easy-Cas9-nls-CDS1

   Plasmid

   #160231

   Purpose

   Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
   

   Use

   Level 0 of moclo golden gate position cds1

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW037

   Plasmid

   #185688

   Purpose

   T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

   Depositor

   Feng Zhang

   Article

   Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

   Insert

   Cas9, Drm1b gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIV495

   Plasmid

   #177702

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS22: pHelper(PmcCAST)_entry_ΔTniQ

   Plasmid

   #168155

   Purpose

   Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

   Plasmid

   #168152

   Purpose

   Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS5: pHelper(AvCAST)_entry_ΔTniQ

   Plasmid

   #168138

   Purpose

   Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

   Plasmid

   #168134

   Purpose

   Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD2 LbCas12a (GB1439)

   Plasmid

   #160573

   Purpose

   Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   LbCas12a
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRWJB006

   Plasmid

   #160113

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   35S

   Available Since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRWJB004

    Plasmid

    #160112

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    UBQ10

    Available Since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GSX2-P2A-tGFP-DTA

    Plasmid

    #161750

    Purpose

    tGFP plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    turboGFP
    

    Use

    CRISPR

    Tags

    P2A-tGFP

    Expression

    Mammalian

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ141-ZmUbi-RZ-Bth

    Plasmid

    #129686

    Purpose

    (Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ141-ZmUbi-RZ-Aa1.2

    Plasmid

    #136373

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ141-ZmUbi-RZ-Bh

    Plasmid

    #136378

    Purpose

    Gateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid

    #133348

    Purpose

    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_blaA and sgRNA_cpaA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh249

    Plasmid

    #106856

    Purpose

    expression of gRNA targeting PIGV

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    PIGV (PIGV Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh101

    Plasmid

    #106772

    Purpose

    expression of gRNA targeting POGLUT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    POGLUT1 (POGLUT1 Human)
    

    Expression

    Mammalian

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. gh246

    Plasmid

    #106855

    Purpose

    expression of gRNA targeting PIGM

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    PIGM (PIGM Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. gh264

    Plasmid

    #106863

    Purpose

    expression of gRNA targeting ALG10B

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ALG10B (ALG10B Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. gh243

    Plasmid

    #106854

    Purpose

    expression of gRNA targeting PIGB

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    PIGB (PIGB Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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