We narrowed to 23,149 results for: REP

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-GA50

Plasmid

#84907

Purpose

induce expression of dipeptide repeat GA in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

GA50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG-TNKS-2-M1054V

Plasmid

#34692

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2-M1054V (TNKS2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

M1054V

Promoter

CMV

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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BE3-P2A-EGFP (pJUL977)

Plasmid

#123612

Purpose

CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3-P2A-EGFP

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCherryGFP-CoV2-WT

Plasmid

#177618

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9-HE-Geminin

Plasmid

#109402

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

HE-Geminin

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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DT40

Plasmid

#80415

Purpose

Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-6.24

Plasmid

#41003

Depositor

James Inglese

Article

Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

Inserts

GSG-P2A-RLuc
4XCRE + Minimal promoter

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pSCBE3

Plasmid

#133966

Purpose

Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

BE3

Use

CRISPR and Synthetic Biology

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAB1619 hU6-BpiI-Cas13bt1-DR

Plasmid

#176314

Purpose

hU6-BpiI-Cas13bt1-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt1 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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VSVGwt-SBP-EGFP_puromycin

Plasmid

#65302

Purpose

VSVG reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-sfGFP

Plasmid

#49645

Purpose

TALEGreen-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

sfGFP

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

Plasmid

#154196

Purpose

Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sgRNA-BC1-A, sgRNA-BC1-C

Use

Lentiviral; Catch barcode activation

Expression

Mammalian

Promoter

hU6 and mU6

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-RCK

Plasmid

#25065

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase domains aa 1329…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pTU1-A-SP44-sfGFP

Plasmid

#163757

Purpose

Expresses Streptomyces codon-optimised sfGFP

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

SP44

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

Plasmid

#32728

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Mutation

-947 AP-1 binding site TGAGTCA deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pIntegrase_5

Plasmid

#60577

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_5

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pJL056

Plasmid

#177168

Purpose

Expresses wildtype MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTcI-Wasabi

Plasmid

#86101

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T

Promoter

pR/pL and pR/pL, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

Plasmid

#214102

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE-Plus

Expression

Mammalian

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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