166,542 results

1. Toronto KnockOut version 3 library (TKOv3)

   Pooled Library

   #90294

   Purpose

   Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

   Depositor

   Jason Moffat

   Article

   Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pMET7-GAG-EGFP

   Plasmid

   #80605

   Purpose

   Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-mCherry

   Plasmid

   #36084

   Purpose

   3rd generation lentiviral plasmid expressing mCherry

   Depositor

   Pantelis Tsoulfas

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFP Cortactin

   Plasmid

   #50728

   Purpose

   mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cortactin (CTTN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Cre:GFP

   Plasmid

   #13776

   Purpose

   Mammalian expression of Cre recombinase fused to GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-GFP fusion protein
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-EGFP

   Plasmid

   #13031

   Purpose

   Mammalian expression vector for cloning your gene fused to EGFP

   Depositor

   Doug Golenbock

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. enIscB

   Plasmid

   #205410

   Purpose

   Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E85R+H369R+S387R+S457R

   Promoter

   CAG, hU6, CMV

   Available Since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV9)

   Viral Prep

   #105545-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   HI

   Tags

   EGFP

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pGEX6P1-mCherry-Nanobody

    Plasmid

    #70696

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    mCherry-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Apple-53BP1trunc

    Plasmid

    #69531

    Purpose

    Fluorescent reporter for DNA damage

    Depositor

    Ralph Weissleder

    Article

    Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    Apple fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti PGK Blast DEST (w524-1)

    Plasmid

    #19065

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-RfxCas13d-HA

    Plasmid

    #141320

    Purpose

    Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-OVA

    Plasmid

    #64599

    Purpose

    Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMitoTimer

    Plasmid

    #52659

    Purpose

    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

    Depositor

    Zhen Yan

    Article

    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

    Insert

    DsRed1-E5
    

    Use

    Dsred

    Tags

    human cytochrome c oxidase subunit VIII mitochond…

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-TREX1

    Plasmid

    #27219

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXLE-hUL

    Plasmid

    #27080

    Purpose

    Integration-free (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    L-MYC (LIN28A, MYCL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238233

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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