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Showing: 2451 - 2500 of 11683 results

  1. pREP41xV5_ccdB

    Plasmid
    #79017
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79017-plasmid-map-sequence-id-151963

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  2. pREP42xV5_ccdB

    Plasmid
    #79018
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79018-plasmid-map-sequence-id-147775

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  3. pJK648

    Plasmid
    #71611
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71611-plasmid-map-sequence-id-131688

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  4. pHL 1904

    Plasmid
    #53021
    Purpose
    AmpR + fimS::gfpLVA::Asp terminator + CamR + ColE1
    Depositor
    Han Lim
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Asp terminator, LVA, and fimS
    Expression
    Bacterial
    Promoter
    fimS
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41393-plasmid-map-sequence-id-364440

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  6. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

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  7. pLIX_403

    Plasmid
    #41395
    Purpose
    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    V5
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41395-plasmid-map-sequence-id-364439

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  9. pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

    Plasmid
    #128450
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128450-plasmid-map-sequence-id-256464

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  10. pMH-Halo tag

    Plasmid
    #154144
    Purpose
    Gateway compatible mammalian expression plasmid for halo tag fusion proteins
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    Halo tag
    Expression
    Mammalian
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154144-plasmid-map-sequence-id-309445

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  11. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

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  12. pRL528

    Plasmid
    #58495
    Purpose
    helper plasmid for bacterial conjugal DNA transfer
    Depositor
    Peter Wolk
    Article
    Elhai et al Methods Enzymol. 1988;167:747-54.
    Insert
    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    Available Since
    Oct. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    58495-plasmid-map-sequence-id-107317

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  15. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  16. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  17. p50 cFlag pcDNA3

    Plasmid
    #20018
    Depositor
    Stephen Smale
    Insert
    Nfkb1 (Nfkb1 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Truncated at the p105 ligation site. It codes for…
    Available Since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20018-plasmid-map-sequence-id-169435

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  18. p52 cFlag pcDNA3

    Plasmid
    #20019
    Depositor
    Stephen Smale
    Insert
    Nfkb2 (Nfkb2 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Truncated at the p100 ligation site. It codes for…
    Available Since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only

  19. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

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  20. pMH-EBFP

    Plasmid
    #161714
    Purpose
    Gateway-compatible destination vector for mammalian expression of N-terminal EBFP protein under CMV promoter
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    161714-plasmid-map-sequence-id-319502

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  21. pAL-hCul1-NTD

    Plasmid
    #29518
    Depositor
    Ning Zheng
    Insert
    CUL1 (CUL1 Human)
    Tags
    Thrombin Cleavage Site
    Expression
    Bacterial
    Mutation
    N-terminal 415 aa
    Available Since
    May 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  22. pCC1R

    Plasmid
    #213497
    Purpose
    Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit
    Depositor
    Mark Isalan
    Inserts
    pcaU
    nahR
    rpaR (rpaR )
    phlF
    cinR
    vanR
    aiiA
    Use
    Synthetic Biology
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    213497-plasmid-map-sequence-id-435615

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  27. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  30. RelA RHD cFlag pcDNA3

    Plasmid
    #20015
    Depositor
    Stephen Smale
    Insert
    RelA (Rela Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Contains only the Rel Homology Domain of RelA. It…
    Available Since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only

  33. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  38. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  39. pHflu2

    Plasmid
    #203880
    Purpose
    pHI1 with insertion of ampicillin-resistance gene
    Depositor
    Bogumil Karas
    Insert
    broad-spectrum class A beta-lactamase TEM-116
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203880-plasmid-map-sequence-id-403830

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  40. PAHA

    Plasmid
    #74663
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PKU1 (PAH Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  49. pBCSK_HIS3_II

    Plasmid
    #109059
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109059-plasmid-map-sequence-id-209551

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  50. pUPD2 nickase D10A Cas9 (GB1690)

    Plasmid
    #160587
    Purpose
    Nickase D10A Cas9 for Nt and Ct fusion.
    Depositor
    Diego Orzaez
    Insert
    nickase D10A Cas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160587-plasmid-map-sequence-id-322079

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Showing: 2451 - 2500 of 11683 results